2016-2019年江苏省南京市人感染副溶血弧菌分子特征分析

吴正燕 董晨 张康娣 谈忠鸣 朱叶飞

吴正燕, 董晨, 张康娣, 谈忠鸣, 朱叶飞. 2016-2019年江苏省南京市人感染副溶血弧菌分子特征分析[J]. 疾病监测. doi: 10.3784/jbjc.202112160417
引用本文: 吴正燕, 董晨, 张康娣, 谈忠鸣, 朱叶飞. 2016-2019年江苏省南京市人感染副溶血弧菌分子特征分析[J]. 疾病监测. doi: 10.3784/jbjc.202112160417
Wu Zhengyan, Dong Chen, Zhang Kangdi, Tan Zhongming, Zhu Yefei. Molecular analysis on Vibrio parahaemolyticus strains isolated from patients in Nanjing, Jiangsu, 2016–2019[J]. Disease Surveillance. doi: 10.3784/jbjc.202112160417
Citation: Wu Zhengyan, Dong Chen, Zhang Kangdi, Tan Zhongming, Zhu Yefei. Molecular analysis on Vibrio parahaemolyticus strains isolated from patients in Nanjing, Jiangsu, 2016–2019[J]. Disease Surveillance. doi: 10.3784/jbjc.202112160417

2016-2019年江苏省南京市人感染副溶血弧菌分子特征分析

doi: 10.3784/jbjc.202112160417
基金项目: 江苏省疾病预防控制中心“科教强业”工程青年人才项目(No. JKRC2016005)
详细信息
    作者简介:

    吴正燕,女,江苏省南京市人,主管技师,主要从事临床检验、病原微生物学研究工作,Email:64950048@qq.com

    通讯作者:

    朱叶飞,Tel:025–58509923,Email:zhuyf@njmu.edu.cn

  • 中图分类号: R211;R378.3

Molecular analysis on Vibrio parahaemolyticus strains isolated from patients in Nanjing, Jiangsu, 2016–2019

Funds: This study was supported by the fund for the Project of Powerful Industry of Science and Education of Jiangsu Provincial Center for Disease Control and Prevention (No. JKRC2016005)
More Information
  • 摘要:   目的  了解江苏省南京市副溶血弧菌临床分离株的分子特征,为其防控提供参考。  方法  收集2016—2019年从腹泻和食物中毒患者分离的副溶血弧菌临床分离株并进行血清型分型,运用聚合酶链式反应(PCR)方法检测菌株的tlhtdhtrhtoxRS/new、ORF8五种毒力基因,采用多位点序列分型(MLST)对菌株进行分子分型分析。  结果  2016—2019年共收集93株临床分离株,菌株优势血清型为O3∶K6(65/93,69.9%),均携带tlh基因,73株属于tdh+trh- toxRS/new+的大流行菌群,非大流行菌群菌株中有5株为tdh-trh-非致病株。 共检出13个ST型,以ST3为主(68/93,73.1%)。  结论  南京地区副溶血弧菌以O3∶K6、ST3型大流行菌群菌株为主,非大流行菌群、非致病株并存。
  • 图  1  基于副溶血弧菌序列型构建的eBURST图

    注:绿色为本研究副溶血弧菌菌株ST型,蓝色为数据库中菌株ST型

    Figure  1.  eBURST diagram of of V. Parahaemolyticus based on STs

    表  1  用于检测副溶血弧菌5种毒力基因的引物

    Table  1.   Primers used for the detection of five virulence genes of V. parahaemolyticus

    目的基因引物名称引物序列(5′~3′)扩增产物长度(bp)参考文献
    tlhtlh-FAAAGCGGATTATGCAGAAGCACTG4507
    tlh-RGCTACTTTCTAGCATTTTCTCTGC
    tdhtdh-FGTAAAGGTCTCTGACTTTTGGAC 2697
    tdh-RTGGAATAGAACCTTCATCTTCACC
    trhtrh-FTTGGCTTCGATATTTTCAGTATCT5007
    trh-RCATAACAAACATATGCCCATTTCCG
    toxRS/newtoxRS/new-FTAATGAGGTAGAAACA6518
    toxRS/new-RACGTAACGGGCCTACA
    ORF8ORF8-FAGGACGCAGTTACGCTTGATG3699
    ORF8-RCTAACGCATTGTCCCTTTGTAG
    下载: 导出CSV

    表  2  副溶血弧菌来源患者的性别和年龄分布

    Table  2.   Sex and age distribution of patients infected with V. parahaemolyticus

    年龄组(岁)男性(例)女性(例)合计(例)
      7~ 1 2 3
     18~473380
     46~ 3 710
      合计514293
    下载: 导出CSV

    表  3  93株副溶血弧菌的毒力基因特征和血清型分布

    Table  3.   Distribution of virulence genes and serotypes of V. parahaemolyticus

     菌株分类菌株数(株)tlhtdhtrhtoxRS/newORF8ST型血清型
    大流行菌群54++++ST3、ST189、ST216、ST332、ST1648O3∶K6、O3∶KUT、O4∶KUT、O4∶K8
    19+++
    非大流行菌群12++ST50、ST189、ST199、ST332、ST345O1∶KUT、O3∶K6、O3∶K8、O3∶KUT、O4∶K8、O4∶KUT、O6∶K18
    1+++
    1++++
    1+++++
    2a++ST1、ST64、ST108、ST190、ST2251O1∶K1、O1∶KUT、O4∶K68
    2a+
    1a++
      注:a. 为非致病菌株
    下载: 导出CSV
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  • 收稿日期:  2020-12-16
  • 网络出版日期:  2021-07-15

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