Abstract: Due to lack of understanding of COVID-19 and its pathogen SARS-CoV-2, nosocomial infections of SARS-CoV-2 occurred in hospitals in the early stage of the COVID-19 epidemic. As the comprehensive institution of disease prevention, control and treatment, the nosocomial infection seriously affected the normal operation of hospitals, so how to effectively prevent and control the nosocomial infection of SARD-CoV-2 is a serious challenge for hospitals. In the fighting against COVID-19 for years, lots about the nosocomial infection prevention and control of the disease have been learned, and after taking targeted measures, the nosocomial infection has been effectively prevented and controlled. The measures include correct pre-hospital examination, triage and case isolation, in-hospital control and self-protection of medical staff, which are essential for the control of the nosocomial infection of SARS-CoV-2. Particularly, in-hospital control can play a very important role in the effective prevention of the nosocomial infection, it should be implemented strictly.