吴春敏, 叶素贞, 胡凤清, 饶晓虹. 2018-2020年福建省南平市沙门菌分子分型及耐药性分析[J]. 疾病监测, 2023, 38(1): 17-22. DOI: 10.3784/jbjc.202202280076
引用本文: 吴春敏, 叶素贞, 胡凤清, 饶晓虹. 2018-2020年福建省南平市沙门菌分子分型及耐药性分析[J]. 疾病监测, 2023, 38(1): 17-22. DOI: 10.3784/jbjc.202202280076
Wu Chunmin, Ye Suzhen, Hu Fengqing, Rao Xiaohong. Molecular typing and drug resistance analysis of Salmonella in Nanping, Fujian, 2018−2020[J]. Disease Surveillance, 2023, 38(1): 17-22. DOI: 10.3784/jbjc.202202280076
Citation: Wu Chunmin, Ye Suzhen, Hu Fengqing, Rao Xiaohong. Molecular typing and drug resistance analysis of Salmonella in Nanping, Fujian, 2018−2020[J]. Disease Surveillance, 2023, 38(1): 17-22. DOI: 10.3784/jbjc.202202280076

2018-2020年福建省南平市沙门菌分子分型及耐药性分析

Molecular typing and drug resistance analysis of Salmonella in Nanping, Fujian, 2018−2020

  • 摘要:
      目的  了解2018—2020年福建省南平市沙门菌血清学分型、分子分型及耐药情况,为沙门菌引起的食源性疾病的溯源和临床合理用药提供指导。
      方法  依据GB 4789.4-2016《食品安全国家标准食品微生物学检验 沙门菌检验》微生物检验方法和《食源性疾病监测工作手册》对收集到的样本进行检测,采用分离培养及生化实验进行沙门菌鉴定,利用玻片凝集法进行血清学试验,利用脉冲场凝胶电泳(PFGE)技术对分离到的菌株进行遗传相关性分析,用微量肉汤稀释法进行耐药性分析。
      结果  55株沙门菌菌株分属21种血清型,以肠炎沙门菌和鼠伤寒沙门菌为主,分别占30.90%和29.09%;食品中分离的沙门菌有19种血清型,食源性疾病病例中分离的沙门菌有4种血清型;菌株的PFGE形成13种带型,具有多态性,相似度为37.29%~100.00%,总体相似度达到 63.77%。 同一血清型可出现不同的PFGE带型,部分肠炎、鼠伤寒、唐卜逊沙门菌出现成簇现象。 沙门菌对氨苄西林、萘啶酸、磺胺异恶唑有较高的耐药性,分别达到67.27%、65.45%、54.55%。 对三代头孢菌素类药物(头孢他啶、头孢西丁)和庆大霉素以及阿奇霉素等药物敏感。 55株沙门菌共发现30种不同的耐药谱,氨苄西林-萘啶酸为优势耐药谱(47.27%,26/55),主要血清型包括肠炎沙门菌和鼠伤寒沙门菌。
      结论  南平市食品中的沙门菌血清型和分子分型多种多样,食品和食源性疾病分离出的沙门菌对15种药物的耐药率较高且种类多样,半数以上菌株存在多重抗生素耐药问题,要继续加强监测。

     

    Abstract:
      Objective   To understand the serological type, molecular type and drug resistance of Salmonella in Nanping, Fujian province, in recent years, and provide technical guidance for the tracing of foodborne diseases caused by Salmonella and the rational clinical use of antibiotics.
      Methods  The samples collected were tested according to “National standard for food safety microbiological test for Salmonella” (GB 4789.4-2016) and “Foodborne disease surveillance work manual”. Salmonella was identified by isolation and biochemical test. Pulsed field gel electrophoresis (PFGE) was conducted to analyze the strains’ genetic relation and micro-broth dilution method was used to test the drug resistance of the strains.
      Results  The 55 Salmonella strains belonged to 21 serotypes, with Salmonella Enterica and Salmonella Typhimurium predominating, accounting for 30.90% and 29.09%, respectively. Nineteen serotypes of Salmonella were isolated from food samples and 4 serotypes from foodborne disease cases. The PFGE bands of the strains were polymorphic with 13 band types. The band similarity ranged from 37.29% to 100.00%, with an overall similarity of 63.77%. Different PFGE band types could be found in the same serotype. Salmonella had high resistance to ampicillin, nalidixic acid and sulfisoxazole with resistance rates of 67.27%, 65.45% and 54.55%, respectively. They were sensitive to three generations of cephalosporins (ceftazidime, cefoxitin), gentamicin and azithromycin. A total of 30 different drug resistance profiles were found in 55 Salmonella strains, with ampicillin-nalidixic acid predominating (47.27%, 26/55), and the main serotypes included Salmonella Enterica and Salmonella Typhimurium.
      Conclusion  Salmonella serotypes in foods in Nanping were diverse. The strains isolated from foods and food-borne disease cases had high and diverse resistance rates to 15 antibiotics, and more than half of the strains were multi-drug resistant, to which close attention needs to be paid.

     

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