2017-2021年重庆市沙坪坝区活禽市场外环境禽流感病毒监测分析

杜文静 王德苗 陈飞 肖小月 唐宇 刘梦颖 刘欣容 周文洁

杜文静, 王德苗, 陈飞, 肖小月, 唐宇, 刘梦颖, 刘欣容, 周文洁. 2017-2021年重庆市沙坪坝区活禽市场外环境禽流感病毒监测分析[J]. 疾病监测, 2023, 38(4): 421-425. doi: 10.3784/jbjc.202210120444
引用本文: 杜文静, 王德苗, 陈飞, 肖小月, 唐宇, 刘梦颖, 刘欣容, 周文洁. 2017-2021年重庆市沙坪坝区活禽市场外环境禽流感病毒监测分析[J]. 疾病监测, 2023, 38(4): 421-425. doi: 10.3784/jbjc.202210120444
Du Wenjing, Wang Demiao, Chen Fei, Xiao Xiaoyue, Tang Yu, Liu Mengying, Liu Xinrong, Zhou Wenjie. Surveillance for avian influenza virus in external environment of live poultry markets in Shapingba district of Chongqing, 2017−2021[J]. Disease Surveillance, 2023, 38(4): 421-425. doi: 10.3784/jbjc.202210120444
Citation: Du Wenjing, Wang Demiao, Chen Fei, Xiao Xiaoyue, Tang Yu, Liu Mengying, Liu Xinrong, Zhou Wenjie. Surveillance for avian influenza virus in external environment of live poultry markets in Shapingba district of Chongqing, 2017−2021[J]. Disease Surveillance, 2023, 38(4): 421-425. doi: 10.3784/jbjc.202210120444

2017-2021年重庆市沙坪坝区活禽市场外环境禽流感病毒监测分析

doi: 10.3784/jbjc.202210120444
详细信息
    作者简介:

    杜文静,女,重庆市人,本科,副主任检验师,主要从事传染病病原学检测工作,Email:1808629770@qq.com

    通讯作者:

    周文洁,Tel:023−65461610,Email:191365027@qq.com

  • 中图分类号: R211; R183.1

Surveillance for avian influenza virus in external environment of live poultry markets in Shapingba district of Chongqing, 2017−2021

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  • 摘要:   目的  了解2017—2021年重庆市沙坪坝区活禽市场外环境中禽流感病毒的分布特点。  方法  在沙坪坝区21个活禽市场随机采集6类环境样本,包括粪便(肛拭)、笼具表面擦拭标本、案板表面擦拭标本、刀具表面擦拭标本、盛装容器面擦拭标本和洗肉水,共1 200份,采用实时聚合酶链式反应(RT-PCR)检测通用甲型流感病毒(Flu A),阳性样本进一步进行H5、H7、H9、N9亚型检测,采用SAS 8.2软件进行统计分析。  结果  样本中Flu A阳性率达68.50%,H5、H7、H9、N9亚型及未分型总阳性率分别为26.33%、0.17%、45.50%、0.00% 及17.17%,差异有统计学意义(χ2=351.563,P<0.001)。 存在H5+H9(19.83%)、H9+N9 (0.67%)和H5+H9+N9(0.67%)混合型。 不同年份、不同季节Flu A阳性率差异有统计学意义(χ2=296.199、 35.350,均P<0.001),Flu A阳性率高峰为第一季度(79.17%)和第二季度(76.67%)。 不同地区活禽市场外环境的Flu A核酸阳性率差异无统计学意义(χ2=11.062,P=0.087);不同环境类型样本中,案板表面擦拭样本Flu A阳性率为72.00%,其次为刀具表面擦拭样本(71.00%),6类环境样本Flu A核酸阳性率差异无统计学意义(χ2= 6.813,P=0.235)。  结论  重庆市沙坪坝区活禽市场环境普遍存在禽流感病毒污染,H9亚型是主要的病原体,混合型占一定比例,污染高峰为每年的第一季度和第二季度。 应重视H9N9和H5H9等新的重配体和变体出现的情况,加强对活禽市场环境监测,切实做好宰杀摆放禽肉的案板和刀具等区域的消毒清洁工作。
  • 表  1  2017-2021年重庆市沙坪坝区涉禽外环境样本甲型流感病毒亚型分布

    Table  1.   Subtype distribution of influenza A virus detected in samples from live poultry-related environment in Shapingba, Chongqing, 2017−2021

    年份样本
    份数
    甲型流
    感病毒
    阳性样本不同亚型分布
    H5H9未分型H5+H9H7+H9H9+N9H5+H9+N9
    2017 120 98(81.67) 2(1.67) 40(33.33) 38(31.67) 16(13.33) 2(1.67) 0(0.00) 0(0.00)
    2018 288 274(95.14) 20(6.94) 92(31.94) 12(4.17) 146(50.69) 0(0.00) 0(0.00) 4(1.39)
    2019 288 238(82.64) 26(9.03) 100(34.72) 36(12.50) 64(22.22) 0(0.00) 8(2.78) 4(1.39)
    2020 216 98(45.37) 4(1.85) 38(17.59) 46(21.30) 10(4.63) 0(0.00) 0(0.00) 0(0.00)
    2021 288 114(39.58) 18(6.25) 20(6.94) 74(25.69) 2(0.69) 0(0.00) 0(0.00) 0(0.00)
    合计 1 200 822(68.50) 70(5.83) 290(24.17) 206(17.17) 238(19.83) 2(0.17) 8(0.67) 8(0.67)
    注:括号外数据为阳性样本数(份),括号内数据为阳性率(%)
    下载: 导出CSV

    表  2  2017-2021年重庆市沙坪坝区不同季度涉禽外环境样本甲型流感病毒亚型分布

    Table  2.   Season specific subtype distribution of influenza A virus detected in samples from poultry-related environment in Shapingba, Chongqing, 2017−2021

    季度样本数(份)甲型流感病毒H5H9未分型H5+H9H7+H9 H9+N9 H5+H9+N9
    第一240190(79.17)22(9.17)60(25.00)24(10.00)68(28.33)0(0.00)8(3.33)8(3.33)
    第二240184(76.67)12(5.00)58(24.17)54(22.50)60(25.00)0(0.00)0(0.00)0(0.00)
    第三336200(59.52)10(2.98)74(22.02)86(25.86)28(8.33)2(0.60)0(0.00)0(0.00)
    第四384248(64.58)26(6.77)98(25.52)42(10.94)82(21.35)0(0.00)0(0.00)0(0.00)
    合计1 200822(68.50)70(5.83)290(2.17)206(17.17)238(19.83)2(0.17)8(1.67)8(1.67)
    注:括号外数据为阳性样本数(份),括号内数据为阳性率(%)
    下载: 导出CSV

    表  3  2017-2021年重庆市沙坪坝区不同地区涉禽外环境样本甲型流感病毒亚型分布

    Table  3.   Area specific subtype distribution of avian influenza A virus detected in samples from poultry-related environment in Shapingba, Chongqing, 2017−2021

     地区
    (镇/街道)
    样本
    份数
    甲型流感
    病毒
    H9+N9H5+H9+N9
    磁器口 240 166(69.17) 0(0.00) 0(0.00)
    青木关 48 24(50.00) 0(0.00) 0(0.00)
    沙坪坝 240 160(66.67) 0(0.00) 4(1.67)
    覃家岗 108 82(75.93) 0(0.00) 0(0.00)
    天星桥 84 58(69.05) 0(0.00) 0(0.00)
    小龙坎 240 164(68.33) 8(3.33) 4(1.67)
    渝碚路 240 168(70.00) 0(0.00) 0(0.00)
    合计 1 200 822(68.50) 8(0.67) 8(0.67)
    注:括号外数据为阳性样本数(份),括号内数据为阳性率(%)
    下载: 导出CSV

    表  4  2017-2021年重庆市沙坪坝区不同类型涉禽外环境样本甲型流感病毒亚型分布

    Table  4.   Sample type specific subtype distribution of influenza A virus detected in poultry-related environment in Shapingba, Chongqing, 2017−2021

     样本类型样本
    份数
    甲型流感病毒H9+N9H5+H9+N9
    案板表面擦拭200144(72.00)0(0.00)2(1.00)
    刀具表面擦拭200142(71.00)2(1.00)2(1.00)
    肛拭子200138(69.00)2(1.00)0(0.00)
    笼具表面擦拭200142(71.00)0(0.00)2(1.00)
    盛装容器表面擦拭200132(66.00)2(1.00)2(1.00)
    洗肉水200124(62.00)2(1.00)0(0.00)
      合计1 200822(68.50)8(0.67)8(0.67)
    注:括号外数据为阳性样本数(份),括号内数据为阳性率(%)
    下载: 导出CSV
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出版历程
  • 收稿日期:  2022-10-12
  • 网络出版日期:  2023-03-27
  • 刊出日期:  2023-04-30

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