3株分离自内蒙古自治区的布鲁氏菌噬菌体生物学特性研究

张宇 朴东日 赵鸿雁 田国忠 范玉 姜海

张宇, 朴东日, 赵鸿雁, 田国忠, 范玉, 姜海. 3株分离自内蒙古自治区的布鲁氏菌噬菌体生物学特性研究[J]. 疾病监测, 2023, 38(4): 451-456. doi: 10.3784/jbjc.202210240464
引用本文: 张宇, 朴东日, 赵鸿雁, 田国忠, 范玉, 姜海. 3株分离自内蒙古自治区的布鲁氏菌噬菌体生物学特性研究[J]. 疾病监测, 2023, 38(4): 451-456. doi: 10.3784/jbjc.202210240464
Zhang Yu, Piao Dongri, Zhao Hongyan, Tian Guozhong, Fan Yu, Jiang Hai. Biological characteristics of three Brucella phages isolated from Inner Mongolia[J]. Disease Surveillance, 2023, 38(4): 451-456. doi: 10.3784/jbjc.202210240464
Citation: Zhang Yu, Piao Dongri, Zhao Hongyan, Tian Guozhong, Fan Yu, Jiang Hai. Biological characteristics of three Brucella phages isolated from Inner Mongolia[J]. Disease Surveillance, 2023, 38(4): 451-456. doi: 10.3784/jbjc.202210240464

3株分离自内蒙古自治区的布鲁氏菌噬菌体生物学特性研究

doi: 10.3784/jbjc.202210240464
基金项目: 国家重点研发计划(No. 2020YFA0907101)
详细信息
    作者简介:

    张宇,女,内蒙古自治区巴彦淖尔市人,硕士研究生在读,主要从事布鲁氏菌噬菌体相关研究工作,Email:zhangyu15847@163.com

    通讯作者:

    姜海,Tel:010–58900767,Email: jianghai@icdc.cn

  • 中图分类号: R211; R378.5

Biological characteristics of three Brucella phages isolated from Inner Mongolia

Funds: This study was supported by the National Key Research & Development Program of China(No. 2020YFA0907101)
More Information
  • 摘要:   目的   对3株分离自内蒙古自治区的布鲁氏菌噬菌体A1、NMY-1和NMY-2生物学特性展开分析,获取生物学信息。  方法  采用双层平板法纯化、增殖噬菌体,观察噬斑特征及测定效价;使用透射电镜观察噬菌体形态;用双层平板法和点滴法测定噬菌体宿主谱、最佳感染复数(MOI)、一步生长曲线和理化稳定性。  结果  经电镜观察,3株噬菌体均为二十面体结构,头部直径61.0 nm~65.6 nm,具有一个长为16.5 nm~19.2 nm短尾;在常规检测浓度(RTD)和104 × RTD下,噬菌体A1和NMY-1可以裂解光滑型牛种、羊种、猪种和沙林鼠种布鲁氏菌,NMY-2能够裂解粗糙型犬种、牛种和羊种布鲁氏菌,且在高浓度(104 × RTD)时可裂解的菌株数量增加;A1、NMY-1和NMY-2的MOI分别为 0.1、0.001、0.01;一步生长曲线结果显示A1和NMY-2潜伏期约为30~60 min,暴发期20~40 min,暴发量分别为2 210 蚀斑形成单位(PFU)/cell和6 000 PFU/cell,NMY-1的潜伏期和暴发期较长,分别为600 min和420 min,暴发量为1.90×105 PFU/cell;3株噬菌体在40~60 ℃,pH为2~13,紫外线照射30 min时保持良好活性。  结论  本研究明确了噬菌体A1、NMY-1和NMY-2的生物学特征和属性,丰富了布鲁氏菌的噬菌体分型系统,为噬菌体疗法治疗耐药布鲁氏菌提供了更多选择。
  • 图  1  噬菌体噬斑

    注:Tb宿主菌为B.abortus 544A,A1宿主菌为B.abortus S19,NMY-1宿主菌为B.melitensis Isfahan,NMY-2宿主菌为B.abortus 45/20

    Figure  1.  Plaques of phages

    图  2  噬菌体在电镜下的形态

    Figure  2.  Phage morphology under transmission electron microscopy

    图  3  噬菌体最佳感染复数及一步生长曲线

    注:A. Tb、A1、NMY-1和NMY-2最佳感染复数的测定结果;B. Tb、A1、NMY-2的生长曲线;C. NMY-1的生长曲线;MOI. 最佳感染复数

    Figure  3.  OMIs and one-step growth curves of phages

    图  4  噬菌体的理化稳定性

    注:A. 噬菌体的热稳定性;B. 噬菌体对紫外线的敏感性;C. 噬菌体对pH的敏感性

    Figure  4.  Physicochemical stability of phages.

    表  1  噬菌体宿主谱测定结果

    Table  1.   Results of phage host profiling

      菌 种菌株数量Tb裂解菌株数量A1裂解菌株数量NMY-1裂解菌株数量NMY-2裂解菌株数量
    RTD104×RTDRTD104×RTDRTD104×RTDRTD104×RTD
    牛种布鲁氏菌(S) 7 7 7 7 7 7 7 0 0
    牛种布鲁氏菌(R) 2 0 0 0 0 0 0 1 2
    羊种布鲁氏菌(S) 11 2 6 5 7 7 11 0 0
    羊种布鲁氏菌(R) 6 0 0 0 0 0 0 3 6
    猪种布鲁氏菌(S) 8 0 8 7 8 5 8 0 0
    犬种布鲁氏菌(R) 11 0 0 0 0 0 0 11 11
    沙林鼠种布鲁氏菌(S) 1 1 1 1 1 1 1 0 0
    注:S. 光滑型;R. 粗糙型;RTD. 常规检测浓度
    下载: 导出CSV
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  • 收稿日期:  2022-10-24
  • 网络出版日期:  2023-04-03
  • 刊出日期:  2023-04-30

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