我国施万菌分布及其分子特征分析

黄振洲 于可艺 汪永禄 李颖 高鹤 白雪梅 肖悦 赖玖连 李坤 王多春

黄振洲, 于可艺, 汪永禄, 李颖, 高鹤, 白雪梅, 肖悦, 赖玖连, 李坤, 王多春. 我国施万菌分布及其分子特征分析[J]. 疾病监测, 2023, 38(4): 384-390. doi: 10.3784/jbjc.202212300556
引用本文: 黄振洲, 于可艺, 汪永禄, 李颖, 高鹤, 白雪梅, 肖悦, 赖玖连, 李坤, 王多春. 我国施万菌分布及其分子特征分析[J]. 疾病监测, 2023, 38(4): 384-390. doi: 10.3784/jbjc.202212300556
Huang Zhenzhou, Yu Keyi, Wang Yonglu, Li Ying, Gao He, Bai Xuemei, Xiao Yue, Lai Jiulian, Li Kun, Wang Duochun. Distribution and molecular characterization of Shewanella in China[J]. Disease Surveillance, 2023, 38(4): 384-390. doi: 10.3784/jbjc.202212300556
Citation: Huang Zhenzhou, Yu Keyi, Wang Yonglu, Li Ying, Gao He, Bai Xuemei, Xiao Yue, Lai Jiulian, Li Kun, Wang Duochun. Distribution and molecular characterization of Shewanella in China[J]. Disease Surveillance, 2023, 38(4): 384-390. doi: 10.3784/jbjc.202212300556

我国施万菌分布及其分子特征分析

doi: 10.3784/jbjc.202212300556
基金项目: 国家科技基础资源调查专项(No. 2021FY100904)
详细信息
    作者简介:

    黄振洲,男,浙江省绍兴市人,博士,主要从事病原生物学相关工作,Email:ronnie_0414@163.com

    通讯作者:

    王多春,Tel:010−58900773,Email:wangduochun@icdc.cn

  • 中图分类号: R211; R378

Distribution and molecular characterization of Shewanella in China

Funds: This study was supported by the National Science and Technology Fundamental Resources Investigation Program of China (No. 2021FY100904)
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  • 摘要:   目的   探索中国施万菌的种群分布、分子流行病学特征及遗传进化关系,为施万菌的流行病学监测和进化研究提供依据。  方法   利用基质辅助激光解吸电离–飞行时间质谱(MALDI-TOF MS)联合gyrB基因测序进行施万菌种水平鉴定;利用7个管家基因(16S rRNA、gyrAgyrBinfBrecNrpoAtopA)进行多位点序列分型(MLST),利用BioNumerics 7.1软件对各个序列类型(ST)构建最小生成树。  结果   本研究共收集全国201株施万菌,鉴定到10个不同的菌种,其中海藻施万菌所占数量最多。 不同分离来源中的施万菌种分布有所差异,临床和食品来源的施万菌种分布相似。 MLST将施万菌实验室分离株划分成136个ST型和15 个克隆复合体(CCs),其中CC12为海藻施万菌优势克隆群。  结论   我国分布的施万菌病原谱丰富多样,提示这些菌株具有高度的遗传多样性。
  • 图  1  施万菌种鉴定及构成分布

    注:A. 施万菌gyrB基因Neighbor-Joining (NJ)进化树。该进化树共纳入10株模式菌株和201株实验室分离株,采用邻接法构建,bootstrap参数设为1 000。施万菌模式菌种用粗体标记,括号里数字分别代表模式菌株和实验室分离株数量。B. 施万菌种构成比

    Figure  1.  Identification and composition distribution of Shewanella spp. collected in this study

    图  2  施万菌分离来源分布

    注:A. 按照临床和环境划分不同来源的施万菌。B. 不同分离来源中的施万菌种分布

    Figure  2.  Distribution of Shewanella spp. isolation sources

    图  3  施万菌分离时间分布

    Figure  3.  Folding Line Chart of isolation year of Shewanella spp.

    图  4  施万菌多位点序列分析最小生成树

    注:A. 按照种水平划分不同菌种的施万菌。B. 按照分离地区划分施万菌。C. 按照分离来源划分施万菌。圆圈内数字代表所属序列类型(sequence type,ST),圆圈大小代表菌株数量多少,CC1~CC15代表本研究鉴定的15个克隆复合体(clonal complexes,CCs)

    Figure  4.  Minimum spanning tree of Shewanella spp. in the multilocus sequence typing analysis

    表  1  多位点序列分析管家基因引物序列信息表

    Table  1.   Summary of housekeeping genes primer sequences in multilocus sequence typing

    基因名称引物名称引物序列 (5'~3')退火温度 (℃)扩增长度(bp)
    16S rRNA 8F AGAGTTTGATCCTGGCTCAG 50 1414
    1492R GGTTACCTTGTTACGACTT
    gyrA gyrA-F TGAAGAACGATTGGAACAARCCNTAYAARAARTC 56 664
    gyrA-R TTTTCAATCAAACGAGCTTTGTTHACYTGRTAHGG
    gyrB gyrB-F GAAGTGGCKATGCAGTGGAA 56 627
    gyrB-R CGRCRAATACCACAGCCRAG
    infB infB-F ATGCCACAGACTATTGAAGCDATYCARCAYGC 56 830
    infB-R GCATCAGCACGAACGTTAAARCCNAYMAKRATNGC
    recN recN-F AGTGAGCATCAACTGACCYTRYTNGAYAGYTAYGC 54 863
    recN-R GGTTGTAAAGGTTGCCCTGGGTTDGTNSWNAC
    rpoA rpoA-F TGGAGCCGCTTGAGCGTGGTTTYGGHCAYAC 56 751
    rpoA-R ATGTAATGAATCGCTTCGGCYTTYARRCAGTT
    topA topA-F GAATTCATCGTTAAGTCGAGYGTDGGBCAYRT 60 860
    topA-R CGCTGGGCCATCATCATGGTYTTYTTNACNCC
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  • [1] Yu KY, Huang ZZ, Xiao Y, et al. Shewanella infection in humans: Epidemiology, clinical features and pathogenicity[J]. Virulence, 2022, 13(1): 1515–1532. DOI:  10.1080/21505594.2022.2117831.
    [2] MacDonell MT, Colwell RR. Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella[J]. Syst Appl Microbiol, 1985, 6(2): 171–182. DOI: 10.1016/S0723−2020(85)80051−5.
    [3] Yousfi K, Bekal S, Usongo V, et al. Current trends of human infections and antibiotic resistance of the genus Shewanella[J]. Eur J Clin Microbiol Infect Dis, 2017, 36(8): 1353–1362. DOI: 10.1007/s10096−017−2962−3.
    [4] Ng WWS, Shum HP, To KKW, et al. Emerging infections due to Shewanella spp. : a case series of 128 cases over 10 years[J]. Front Med, 2022, 9: 850938. DOI:  10.3389/fmed.2022.850938.
    [5] Chia-Wei L, Cheng JF, Tung KC, et al. Evolution of trimethoprim/sulfamethoxazole resistance in Shewanella algae from the perspective of comparative genomics and global phylogenic analysis[J]. J Microbiol Immunol Infect, 2022, 55(6 Pt 2): 1195–1202. DOI:  10.1016/j.jmii.2021.09.014.
    [6] Holt HM, Gahrn-Hansen B, Bruun B. Shewanella algae and Shewanella putrefaciens: clinical and microbiological characteristics[J]. Clin Microbiol Infect, 2005, 11(5): 347–352. DOI: 10.1111/j.1469−0691.2005.01108.x.
    [7] Antonelli A, Di Palo DM, Galano A, et al. Intestinal carriage of Shewanella xiamenensis simulating carriage of OXA-48-producing enterobacteriaceae[J]. Diagn Microbiol Infect Dis, 2015, 82(1): 1–3. DOI:  10.1016/j.diagmicrobio.2015.02.008.
    [8] Zong ZY. Nosocomial peripancreatic infection associated with Shewanella xiamenensis[J]. J Med Microbiol, 2011, 60(Pt 9): 1387–1390. DOI:  10.1099/jmm.0.031625-0.
    [9] Janda JM, Abbott SL. The genus Shewanella: from the briny depths below to human pathogen[J]. Crit Rev Microbiol, 2014, 40(4): 293–312. DOI:  10.3109/1040841X.2012.726209.
    [10] Sterniša M, Bucar F, Kunert O, et al. Targeting fish spoilers Pseudomonas and Shewanella with oregano and nettle extracts[J]. Int J Food Microbiol, 2020, 328: 108664. DOI:  10.1016/j.ijfoodmicro.2020.108664.
    [11] Martín-Rodríguez AJ, Suárez-Mesa A, Artiles-Campelo F, et al. Multilocus sequence typing of Shewanella algae isolates identifies disease-causing Shewanella chilikensis strain 6I4[J]. FEMS Microbiol Ecol, 2019, 95(1): fiy210. DOI:  10.1093/femsec/fiy210.
    [12] Maiden MCJ. Multilocus sequence typing of bacteria[J]. Annu Rev Microbiol, 2006, 60: 561–588. DOI:  10.1146/annurev.micro.59.030804.121325.
    [13] Fang YJ, Wang YL, Liu ZD, et al. Multilocus sequence analysis, a rapid and accurate tool for taxonomic classification, evolutionary relationship determination, and population biology studies of the genus Shewanella[J]. Appl Environ Microbiol, 2019, 85(11): e03126–18. DOI: 10.1128/AEM.03126−18.
    [14] Yu KY, Huang ZZ, Li Y, et al. Establishment and application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for detection of Shewanella genus[J]. Front Microbiol, 2021, 12: 625821. DOI:  10.3389/fmicb.2021.625821.
    [15] 蔡红艳, 方玉洁, 于可艺, 等. 基于16S rRNAgyrB基因的施万菌种水平鉴定分析[J]. 疾病监测,2021,36(1):42–47. DOI: 10.3784/jbjc.202007130239.

    Cai HY, Fang YJ, Yu KY, et al. Identification of Shewanella at species level based on16S rRNA and gyrB genes[J]. Dis Surveill, 2021, 36(1): 42–47. DOI:  10.3784/jbjc.202007130239.
    [16] Lemaire ON, Mejean V, Iobbi-Nivol C. The Shewanella genus: ubiquitous organisms sustaining and preserving aquatic ecosystems[J]. FEMS Microbiol Rev, 2020, 44(2): 155–170. DOI:  10.1093/femsre/fuz031.
    [17] Maiden MCJ, Bygraves JA, Feil E, et al. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms[J]. Proc Natl Acad Sci USA, 1998, 95(6): 3140–3145. DOI:  10.1073/pnas.95.6.3140.
    [18] Gogarten JP, Doolittle WF, Lawrence JG. Prokaryotic evolution in light of gene transfer[J]. Mol Biol Evol, 2002, 19(12): 2226–2238. DOI:  10.1093/oxfordjournals.molbev.a004046.
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  • 收稿日期:  2022-12-30
  • 网络出版日期:  2023-03-14
  • 刊出日期:  2023-04-30

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