齐可欣, 易雪丽, 王鸣柳, 王建平, 孙晖, 郑翰. 副猪链球菌临床分离株引起小鼠大脑炎性反应的特征及机制研究[J]. 疾病监测, 2023, 38(3): 351-357. DOI: 10.3784/jbjc.202301020558
引用本文: 齐可欣, 易雪丽, 王鸣柳, 王建平, 孙晖, 郑翰. 副猪链球菌临床分离株引起小鼠大脑炎性反应的特征及机制研究[J]. 疾病监测, 2023, 38(3): 351-357. DOI: 10.3784/jbjc.202301020558
Qi Kexin, Yi Xueli, Wang Mingliu, Wang Jianping, Sun Hui, Zheng Han. Characteristics and mechanism of cerebral inflammatory response induced by Streptococcus parasuis clinical strains in mice[J]. Disease Surveillance, 2023, 38(3): 351-357. DOI: 10.3784/jbjc.202301020558
Citation: Qi Kexin, Yi Xueli, Wang Mingliu, Wang Jianping, Sun Hui, Zheng Han. Characteristics and mechanism of cerebral inflammatory response induced by Streptococcus parasuis clinical strains in mice[J]. Disease Surveillance, 2023, 38(3): 351-357. DOI: 10.3784/jbjc.202301020558

副猪链球菌临床分离株引起小鼠大脑炎性反应的特征及机制研究

Characteristics and mechanism of cerebral inflammatory response induced by Streptococcus parasuis clinical strains in mice

  • 摘要:
      目的  研究副猪链球菌临床分离株引起小鼠大脑炎性反应的特征及其机制。
      方法  副猪链球菌临床分离株NN1和BS26及高致病型猪链球菌P1/7感染C57BL/6小鼠后,观察其脑组织中的细菌载量,并提取脑组织RNA,使用实时荧光定量聚合酶链式反应方法明确诱导型一氧化氮合酶(iNOS)、促炎性细胞因子IL-1β以及核苷酸结合寡聚化结构域样受体(NOD1/2)表达的时间动力学特征。 副猪链球菌临床分离株NN1和BS26及猪链球菌P1/7分别与小鼠原代星型胶质细胞及小胶质细胞细胞系BV2细胞相互作用后,分别测定细胞上清液中一氧化氮(NO)浓度以及IL-1β和NOD1/2 mRNA转录水平的时间动力学特征。
      结果  副猪链球菌临床分离株感染小鼠后可在小鼠脑组织中持续存在超过48 h,并在感染早期引起小鼠脑组织中iNOS、IL-1β及NOD1/2受体转录水平的显著升高(P<0.05)。 小胶质细胞可能在副猪链球菌临床分离株诱导脑组织产生NO和IL-1β中发挥重要作用。 副猪链球菌通过NOD1/2受体激活星形胶质细胞的能力显著强于小胶质细胞(P<0.05)。
      结论  副猪链球菌临床分离株NN1和BS26在感染早期通过产生大量NO和IL-1β等炎性介质引起小鼠大脑炎性反应和损伤,NOD1/2受体参与了副猪链球菌临床分离株诱导的小鼠大脑炎性反应。星形胶质细胞和小胶质细胞在副猪链球菌临床分离株诱导的上述炎性反应中发挥了重要作用,且副猪链球菌激活星形胶质细胞和小胶质细胞的机制存在差异。

     

    Abstract:
      Objective  To understand the characteristics and mechanism of the cerebral inflammatory response induced by Streptococcus parasuis clinical strains in mice.
      Methods  The bacterial loads, kinetics of inducible nitric oxide synthase (iNOS), pro-inflammatory cytokine IL-1β and NOD-like receptor (NOD1/2) transcription level in the brain of mice infected with clinical S. parasuis strain NN1, S. parasuis clinical strain BS26 and highly pathogenic S. suis strain P1/7 were analyzed with quantitative polymerase chain raction. The NO concentration, IL-1β and NOD1/2 mRNA transcription levels of the murine primary astrocyte and microglia cell line BV2 cell infected with aforementioned three strains were also evaluated.
      Results  S. parasuis clinical strains NN1 and BS26 persisted in the brain of infected mice for more than 48 hours. They could upregulate the transcription level of iNOS, IL-1β and NOD1/2 mRNA in the brain of infected mice at the early phase of infection. Microglia cells might play a more important role to induce the production of NO and IL-1β in the brains of mice infected with S. parasuis clinical strains NN1 and BS26. NOD1/2 receptors were crucial for the activation of astrocytes by S. parasuis clinical strains NN1 and BS26.
      Conclusion  S. parasuis clinical strains NN1 and BS26 could induce the cerebral inflammatory responses at the very early phase of infection via NOD1/2 receptors. Astrocytes and microglia played an important role in the cerebral inflammatory response induced by the infection of S. parasuis clinical strains on mice. In addition, the mechanism to activate astrocyte and microglia cells by the infection of S. parasuis clinical strains varied.

     

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