中国福氏志贺菌前噬菌体分布与基因组进化研究

梁俊容 金东 杨晶 卢珊 熊衍文 叶长芸 徐建国

梁俊容, 金东, 杨晶, 卢珊, 熊衍文, 叶长芸, 徐建国. 中国福氏志贺菌前噬菌体分布与基因组进化研究[J]. 疾病监测. doi: 10.3784/jbjc.202301060564
引用本文: 梁俊容, 金东, 杨晶, 卢珊, 熊衍文, 叶长芸, 徐建国. 中国福氏志贺菌前噬菌体分布与基因组进化研究[J]. 疾病监测. doi: 10.3784/jbjc.202301060564
Liang Junrong, Jin Dong, Yang Jing, Lu Shan, Xiong Yanwen, Ye Changyun, Xu Jianguo. Prophages distribution and genome evolution of Shigella flexneri in China[J]. Disease Surveillance. doi: 10.3784/jbjc.202301060564
Citation: Liang Junrong, Jin Dong, Yang Jing, Lu Shan, Xiong Yanwen, Ye Changyun, Xu Jianguo. Prophages distribution and genome evolution of Shigella flexneri in China[J]. Disease Surveillance. doi: 10.3784/jbjc.202301060564

中国福氏志贺菌前噬菌体分布与基因组进化研究

doi: 10.3784/jbjc.202301060564
基金项目: 国家重点研发计划项目(No. 2021YFC0863400,No. 2021YFC2302000);未知细菌发现与功能研究单位(No. 2018RU010)
详细信息
    作者简介:

    梁俊容,女,湖南省娄底市人,博士,主要从事肠道致病菌研究,Email:liangjunrong@icdc.cn

    通讯作者:

    徐建国,Tel:010–58900701,Email:xujianguo@icdc.cn

  • 中图分类号: R211; R378.2

Prophages distribution and genome evolution of Shigella flexneri in China

Funds: This study was supported by National Key Research and Development Program of China (No. 2021YFC0863400, No. 2021YFC2302000) and the Unknown Bacterial Discovery and Function Research Unit (No. 2018RU010)
  • 摘要:   目的  前噬菌体是细菌基因组中重要可移动原件,在细菌毒力和宿主适应性方面发挥着重要作用。 由噬菌体介导的福氏志贺菌血清型转化频繁发生,给志贺菌的监测、诊断和预防工作带来不便。 为了探究我国福氏志贺菌基因组进化与其前噬菌体相关的血清型别转换之间的关系,利用生物信息学方法将前噬菌体在福氏志贺菌基因组中的分布与进化进行关联分析。   方法  在中国17省(市、自治区)选取的294株福氏志贺菌具有丰富的血清型多态性。 通过比较不同血清型福氏志贺菌基因组中原噬菌体及血清型转换噬菌体的覆盖度,统计分析其分布规律。   结果  部分菌株可携带多种前噬菌体,同一种前噬菌体也可分布在不同血清型菌株中;部分菌株虽携带血清型转换噬菌体却并未发生血清型转换事件。 不同血清型转换噬菌体在福氏志贺菌进化树中成簇出现,表明该部分菌株出现了血清型集中转换现象。   结论  本研究有助于揭示前噬菌体的遗传多样性及与福氏志贺菌进化和血清型变异之间的关系,对预防和控制志贺菌的感染具有重要意义。
  • 图  1  不同血清型福氏志贺菌前噬菌体分布

    注:热图顶端颜色标签按照294株福氏志贺菌不同的血清型划分。热图内部颜色代表菌株中含有右侧噬菌体的个数,具体个数见左侧颜色标尺

    Figure  1.  Distribution of prophages among Shigella flexneri with different serotypes

    图  2  不同血清型福氏志贺菌基因组中志贺菌相关前噬菌体分布

    注:热图顶端颜色标签按照294株福氏志贺菌不同的血清型划分。热图内部颜色代表菌株中含有右侧噬菌体的个数,具体见左侧颜色标尺

    Figure  2.  Distribution of Shigella associated prophages among Shigella flexneri with different serotypes

    图  3  福氏志贺菌进化树与前噬菌体分布情况

    注:最内圈表述菌株宿主信息,第二圈表示菌株血清型分类。外圈热图由外向内分别表示噬菌体sfx、Stx2-converting_phage_86、Stx2-converting_phage_1717、Stx1_converting_phage_DNA、Shigella_phage_Ss-VASD、Shigella_phage_SfII,Shigella_phage_Sf6、Shigella_phage_SHSML-45、Shigella_phage_POCJ13、Shigella_phage_75_02_Stx、Enterobacteria_phage_SfV、Enterobacteria_phage_SfI、Enterobacteria_phage_Sf6及Enterobacteria_phage_Sf101在福氏志贺菌基因组的比对相似度

    Figure  3.  Maximum likelihood tree of the 294 isolates and distribution of prophages

    表  1  不同省(市、自治区)挑选的福氏志贺菌血清型分布

    Table  1.   Serotypes distribution of Shigella flexneri from different provinces

    血清型省  份总计
    安徽北京福建甘肃(动物菌株+人菌株)贵州黑龙江河南湖北江苏辽宁青海陕西上海山西四川西藏浙江
    1a 2 1 1 4+0 1 1 15 1 1 4 7 38
    1b 2+2 3 1 8
    1d 1 2 1 4
    2a 4 1 4 5+4 4 4 17 1 3 1 6 1 55
    2b 2 2 2 15+0 1 2 8 1 3 2 7 3 1 1 49
    3a 0+2 2 1 3 1 9
    4a 1 1
    4av 1 1
    4b 1 1
    7b 1 1
    X 2 0+2 1 2 11 18
    Xv 12 3 5 8+15 3 22 2 1 2 3 3 1 11 1 1 93
    Y 1 0+1 1 4 7
    Y-gtrI 1 1
    Y-gtrII 1 1 1 3
    Yv 1 1
    Yv-gtrII 1 2 3
    合计 25 8 13 60(34+26) 11 14 91 6 9 4 15 3 1 27 2 3 2 294
    注:-. 表示该省(市、自治区)没有分离到该血清型菌株
    下载: 导出CSV
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  • 收稿日期:  2023-01-06
  • 录用日期:  2023-04-27
  • 网络出版日期:  2023-04-27

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