2014-2019年山东省潍坊市流行的肠道病毒A组71型的VP1区基因特征分析

张凤娟 刘德辉 王晓倩 霍锡元 张作栋 聂清

张凤娟, 刘德辉, 王晓倩, 霍锡元, 张作栋, 聂清. 2014-2019年山东省潍坊市流行的肠道病毒A组71型的VP1区基因特征分析[J]. 疾病监测, 2023, 38(10): 1229-1232. doi: 10.3784/jbjc.202302090030
引用本文: 张凤娟, 刘德辉, 王晓倩, 霍锡元, 张作栋, 聂清. 2014-2019年山东省潍坊市流行的肠道病毒A组71型的VP1区基因特征分析[J]. 疾病监测, 2023, 38(10): 1229-1232. doi: 10.3784/jbjc.202302090030
Zhang Fengjuan, Liu Dehui, Wang Xiaoqian, Huo Xiyuan, Zhang Zuodong, Nie Qing. Genetic characterization of VP1 region of enterovirus group A 71 circulating in Weifang, Shandong, 2014−2019[J]. Disease Surveillance, 2023, 38(10): 1229-1232. doi: 10.3784/jbjc.202302090030
Citation: Zhang Fengjuan, Liu Dehui, Wang Xiaoqian, Huo Xiyuan, Zhang Zuodong, Nie Qing. Genetic characterization of VP1 region of enterovirus group A 71 circulating in Weifang, Shandong, 2014−2019[J]. Disease Surveillance, 2023, 38(10): 1229-1232. doi: 10.3784/jbjc.202302090030

2014-2019年山东省潍坊市流行的肠道病毒A组71型的VP1区基因特征分析

doi: 10.3784/jbjc.202302090030
基金项目: 潍坊市卫生健康委员会项目(No. wfwsjk_2019_045)
详细信息
    作者简介:

    张凤娟,女,山东省潍坊市人,硕士,主管技师,主要从事病原微生物的相关工作,Email:wfcdczfj@163.com

    通讯作者:

    聂清,Tel:0536–8098503,Email:nieqing0454@163.com

  • 中图分类号: R211;R373.2

Genetic characterization of VP1 region of enterovirus group A 71 circulating in Weifang, Shandong, 2014−2019

Funds:  This study was supported by the fund for the Project of Health Commission of Weifang (No. wfwsjk_2019_045)
More Information
  • 摘要:   目的  了解2014—2019年山东省潍坊市流行的肠道病毒A组71型(EV71)VP1区基因遗传特征、进化关系及VP1区密码子所处的选择性压力。  方法  随机选取2014—2019年潍坊市EV71感染手足口病患者的阳性标本,经人横纹肌肉瘤细胞分离培养后提取病毒总RNA,采用反转录聚合酶链式反应对VP1区基因扩增后进行序列测定,并分析与EV71各基因型代表株的核苷酸及氨基酸的同源性,构建2008年以来我国不同省份EV71及本地EV71 VP1区系统进化树。 通过Datamonkey 在线服务器采用单似然祖先计数(SLAC)及固定效应可能性模型(FEL)方法对C4a亚型 VP1区密码子进行选择性压力分析。  结果  共获得的41株本地EV71毒株,均与C4a基因亚型的同源性最高,核苷酸同源性为94.9%~98.2%,氨基酸同源性为98.3%~99.6%。 系统进化树显示本地EV71毒株均属C4a基因亚型,形成2个明显的支系,每一分支中既有成簇毒株也有散在分布毒株,并与多地毒株亲缘关系较近。 采用SLAC和FEL分别鉴定出136和190个密码子为负向选择性压力位点,不存在正向选择性压力位点。  结论  2014—2019年潍坊市流行的EV71毒株属于C4a基因亚型,存在多条传播链,VP1区强烈的负选择压力表明 VP1区抗原的稳定性,VP1蛋白的变异不是由宿主免疫系统的选择性压力引起的,这有利于EV71疫苗的应用。
  • 图  1  基于VP1区构建的189株肠道病毒 A 组 71 型毒株的最大似然系统进化树

    注:本地分离株采用★标记;不同基因亚型及本地分离株形成的2个分支用不同颜色表示,其中:●为A基因亚型; ●为E、D及G基因亚型; ●为F基因亚型; ●为B基因亚型; ●为C1、C2、C3及C5基因亚型; ●为C4b基因亚型; ●为C4a基因亚型; ●为分支1; ●为分支2

    Figure  1.  Phylogenetic tree of 189 EV71 strains based on VP1 gene constructed by using Maximum Likelihood method

    表  1  41株本地毒株与肠道病毒 A 组 71 型各基因型代表株的核苷酸及氨基酸序列同源性

    Table  1.   Homology of nucleotide and amino acid between 41 local strains and reference EV71 strains with different genotypes

    参考株基因型核苷酸同源性(%)氨基酸同源性(%)
    U22521A80.3~82.493.6~95.2
    U22522B83.1~85.296.2~97.6
    AF135945C187.8~90.197.6~98.6
    AF135941C287.4~89.397.5~98.5
    AB115494C386.9~89.497.9~98.9
    JQ514785C4a94.9~98.298.3~99.6
    AF302996C4b89.8~92.196.9~97.9
    AM490141C585.4~87.796.2~97.3
    AY179600D78.7~80.493.9~94.6
    JN255590E81.9~83.094.9~96.2
    HG421068F81.5~82.995.2~96.2
    KF906417G81.8~83.096.6~97.9
    下载: 导出CSV
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  • 收稿日期:  2023-02-09
  • 网络出版日期:  2023-08-17
  • 刊出日期:  2023-11-07

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