一株同时携带blaCTX-M-14blaCTX-M-15基因的多重耐药宋内志贺菌耐药基因及质粒分析

刘康康 李春晓

刘康康, 李春晓. 一株同时携带blaCTX-M-14和blaCTX-M-15基因的多重耐药宋内志贺菌耐药基因及质粒分析[J]. 疾病监测. doi: 10.3784/jbjc.202302130035
引用本文: 刘康康, 李春晓. 一株同时携带blaCTX-M-14blaCTX-M-15基因的多重耐药宋内志贺菌耐药基因及质粒分析[J]. 疾病监测. doi: 10.3784/jbjc.202302130035
Liu Kangkang, Li Chunxiao. Resistance genes and plasmids of a multi-drug resistant Shigella sonnei strain carrying both blaCTX-M-14 and blaCTX-M-15 genes[J]. Disease Surveillance. doi: 10.3784/jbjc.202302130035
Citation: Liu Kangkang, Li Chunxiao. Resistance genes and plasmids of a multi-drug resistant Shigella sonnei strain carrying both blaCTX-M-14 and blaCTX-M-15 genes[J]. Disease Surveillance. doi: 10.3784/jbjc.202302130035

一株同时携带blaCTX-M-14blaCTX-M-15基因的多重耐药宋内志贺菌耐药基因及质粒分析

doi: 10.3784/jbjc.202302130035
详细信息
    作者简介:

    刘康康,男,河南省周口市人,硕士研究生在读,主要从事病原菌基因组进化研究,Email:conquerleo@163.com

    通讯作者:

    李春晓,Tel:010–66948592;Email:lichunxiao@bmi.ac.cn

  • 中图分类号: R211; R378

Resistance genes and plasmids of a multi-drug resistant Shigella sonnei strain carrying both blaCTX-M-14 and blaCTX-M-15 genes

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  • 摘要:   目的  对1株同时耐头孢菌素和阿奇霉素的多重耐药宋内志贺菌进行全基因组测序分析,明确其携带的耐药基因和质粒特征。  方法  通过对2011年上海市的60株宋内志贺菌进行抗菌药物敏感性分析,筛选出1株同时耐头孢菌素和阿奇霉素的菌株Sh11sh529,使用二代和纳米孔测序技术获得其基因组序列,分析基因组的耐药基因、质粒、可移动遗传元件等。  结果  Sh11sh529基因组中检测到了11个耐药基因以及gyrA基因喹诺酮耐药决定区(QRDR)的突变位点gyrA-S83L,耐药基因包括2种CTX-M型超广谱β内酰胺酶(ESBL)基因blaCTX-M-14blaCTX-M-15,其中blaCTX-M-14mphAaac(3)-IIddfrA17aadA5sul1qacEdelta基因由1个IncB/O/K/Z型质粒pSh11sh529-2携带,blaCTX-M-14上游有1个ISEcp1插入子,mphA上游有1个IS26插入子。 而blaCTX-M-15由IncFII型质粒pSh11sh529-3携带,在其上游有1个ISEcp1插入子。 该菌株表现出多重耐药性,对头孢菌素类、大环内酯类、氨基糖苷类、磺胺类、青霉素类等5类抗菌药物中的9种耐药。  结论  应当加强对多重耐药菌株以及同时携带多个类型ESBL菌株的监测和研究,以制定策略减少其流行和传播。
  • 图  1  pSh11sh529-2及相似质粒比较圈图

    注:红色色块代表耐药基因,橙色代表插入子,紫色为整合子

    Figure  1.  Circular comparisons image of pSh11sh529-2 and similar plasmids

    图  2  pSh11sh529-3及相似质粒比较圈图

    注:红色色块代表耐药基因,橙色代表插入子,灰色为假定蛋白

    Figure  2.  Circular comparisons image of pSh11sh529-3 and similar plasmid

    图  3  整合子结构图

    注:箭头长度与基因长度成比例,箭头方向代表基因的方向

    Figure  3.  Structure of integrons

    表  1  Sh11sh529的药物敏感性

    Table  1.   Drug susceptibility of strain Sh11sh529

    抗菌药物种类抗菌药物MIC
    (μg/mL)
    药物
    敏感性
    氨基糖苷类 妥布霉素 ≥16 耐药
    庆大霉素 ≥16 耐药
    阿米卡星 ≤16 敏感
    碳青霉烯类 亚胺培南 ≤4 敏感
    头孢菌素类 头孢曲松 ≥64 耐药
    头孢他啶 16 中等
    头孢西丁 ≤8 敏感
    头孢哌酮 ≥64 耐药
    头孢吡肟 ≤8 敏感
    头孢唑啉 ≥32 耐药
    氟喹诺酮类 诺氟沙星 ≤4 敏感
    左氧氟沙星 ≤2 敏感
    叶酸通路抑制剂 甲氧苄胺嘧啶/磺胺甲基异恶唑 ≥4/76 耐药
    大环内酯类 阿奇霉素 ≥32 耐药
    单环β内酰胺类 氨曲南 16 中等
    硝基呋喃 呋喃妥因 ≤32 敏感
    青霉素类 替卡西林 ≥128 耐药
    哌拉西林 ≥128 耐药
    氨苄西林 ≥32 耐药
    氯霉素类 氯霉素 ≤8 敏感
    四环素类 四环素 ≤4 敏感
    β-内酰胺类/ β-内酰胺酶抑制剂 替卡西林/克拉维酸 32/264/2 中等
    注:MIC. 最小抑菌浓度
    下载: 导出CSV

    表  2  Sh11sh529携带的主要耐药基因

    Table  2.   Main resistance genes carried by strain Sh11sh529

     序列类型起始位点终止位点耐药基因匹配度(%)覆盖度(%)
    染色体 1 962 708 1 963 496 aadA1 99.36 100.00
    染色体 1 963 554 1 964 078 sat-2 100.00 100.00
    染色体 1 964 173 1 964 606 dfrA1 99.62 99.62
    pSh11sh529-2 110 933 111 808 blaCTX-M-14 100.00 100.00
    pSh11sh529-2 54 479 55 078 dfrA17 100.00 100.00
    pSh11sh529-2 53 560 54 348 aadA5 100.00 100.00
    pSh11sh529-2 53 007 53 354 qacEdelta1 100.00 100.00
    pSh11sh529-2 52 174 53 013 sul1 100.00 100.00
    pSh11sh529-2 47 555 48 415 aac(3)-IId 100.00 100.00
    pSh11sh529-2 43 130 44 035 mphA 100.00 100.00
    pSh11sh529-3 61 400 62 275 blaCTX-M-15 100.00 100.00
    下载: 导出CSV
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  • 收稿日期:  2023-02-13
  • 网络出版日期:  2023-05-05

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