陶霞, 张晶, 王安娜, 周勇, 侯水平, 吴新伟. 广东省广州市鼠形动物携带新埃立克体的分子特征分析[J]. 疾病监测, 2023, 38(11): 1371-1376. DOI: 10.3784/jbjc.202303070088
引用本文: 陶霞, 张晶, 王安娜, 周勇, 侯水平, 吴新伟. 广东省广州市鼠形动物携带新埃立克体的分子特征分析[J]. 疾病监测, 2023, 38(11): 1371-1376. DOI: 10.3784/jbjc.202303070088
Tao Xia, Zhang Jing, Wang Anna, Zhou Yong, Hou Shuiping, Wu Xinwei. Molecular characteristics of Neoehrlichia mikurensis carried by rodents in Guangzhou, Guangdong[J]. Disease Surveillance, 2023, 38(11): 1371-1376. DOI: 10.3784/jbjc.202303070088
Citation: Tao Xia, Zhang Jing, Wang Anna, Zhou Yong, Hou Shuiping, Wu Xinwei. Molecular characteristics of Neoehrlichia mikurensis carried by rodents in Guangzhou, Guangdong[J]. Disease Surveillance, 2023, 38(11): 1371-1376. DOI: 10.3784/jbjc.202303070088

广东省广州市鼠形动物携带新埃立克体的分子特征分析

Molecular characteristics of Neoehrlichia mikurensis carried by rodents in Guangzhou, Guangdong

  • 摘要:
      目的  调查广东省广州市鼠形动物携带新埃立克体的情况,并分析其分子特征。
      方法  使用夹夜法和笼夜法在广州市9个区捕获鼠形动物,解剖获得鼠脾,通过巢式聚合酶链式反应(PCR)扩增16S rRNA和groEL基因,纯化、测序后进行序列比对和同源性分析。
      结果  捕获广州市鼠形动物1 104只,其中29只新埃立克体检测阳性,总阳性率2.63%。 2019、2020、2021年捕鼠数分别为510、307、287只,阳性率分别是0.39%、0.98%、8.36%。 阳性鼠形动物集中于广州市从化区(9.00%)、增城区(3.23%)、白云区(0.86%)。 调查的6种鼠形动物中,以黄胸鼠(7.69%)检出率最高,其次为褐家鼠(2.87%)、黄毛鼠(1.11%)。 系统进化分析显示广州市新埃立克体的groEL与16S rRNA基因序列均高度同源,属于Cluster Ⅳ这一进化支。
      结论  广州市鼠形动物存在一定比例的新埃立克体检出,且检出阳性的区域集中,基因序列高度同源,可能存在新埃立克体的潜在爆发风险,需进一步加强其分子流行病学监测工作。

     

    Abstract:
      Objective   To analyze the molecular characteristics of Neoehrlichia mikurensis isolated from rodents in Guangzhou, Guangdong province.
      Methods  Rodents were captured by night trapping and caging methods in 9 districts in Guangzhou, the spleens of rodents captured were dissected, and nested polymerase chain reaction (PCR) was applied to amplify the fragments of the 16S rRNA and groEL genes of N. mikurensis. In addition, the positive PCR products were sequenced and phylogenetic analysis was performed by using the corresponding sequences from GenBank.
      Results   In 1104 rodent samples, 29 were tested to be positive for N. mikurensis, the overall positive rate was 2.63%. The positive rate was 0.39% (2/510) in 2019, 0.98% (3/307) in 2020, and 8.36% (24/287) in 2021, the differences were significant. The positive rodents were mainly detected in Conghua (9.00%), Zengcheng (3.23%) and Baiyun districts (0.86%). In six rodent species collected, Rattus flavipectus had the highest positive rate (7.69%), followed by Rattus norvegicus (2.87%) and Rattus losea (1.11%). The sequences of the groEL and 16S rRNA genes of N. mikurensis in Guangzhou were highly homologous, belonging to Cluster Ⅳ clade.
      Conclusion   N. mikurensis strains, with high homologous gene sequences, have been detected in rodents in Guangzhou with the high detection rate in some districts, indicating a potential risk for N. mikurensis infection outbreak. It is necessary to strengthen the molecular epidemiology surveillance for N. mikurensis in Guangzhou.

     

/

返回文章
返回