2015-2021年江苏省徐州市住院儿童严重急性呼吸道感染病例鼻病毒感染分析

曹润冬 朱人和 宋娟 夏冬 宋芹芹 夏志强 刘宓 杜海军 韩俊 高晨

曹润冬, 朱人和, 宋娟, 夏冬, 宋芹芹, 夏志强, 刘宓, 杜海军, 韩俊, 高晨. 2015-2021年江苏省徐州市住院儿童严重急性呼吸道感染病例鼻病毒感染分析[J]. 疾病监测, 2023, 38(10): 1205-1210. doi: 10.3784/jbjc.202305220245
引用本文: 曹润冬, 朱人和, 宋娟, 夏冬, 宋芹芹, 夏志强, 刘宓, 杜海军, 韩俊, 高晨. 2015-2021年江苏省徐州市住院儿童严重急性呼吸道感染病例鼻病毒感染分析[J]. 疾病监测, 2023, 38(10): 1205-1210. doi: 10.3784/jbjc.202305220245
Cao Rundong, Zhu Renhe, Song Juan, Xia Dong, Song Qinqin, Xia Zhiqiang, Liu Mi, Du Haijun, Han Jun, Gao Chen. Rhinovirus infection among severe acute respiratory infection cases in hospitalized children in Xuzhou city, Jiangsu province, 2015−2021[J]. Disease Surveillance, 2023, 38(10): 1205-1210. doi: 10.3784/jbjc.202305220245
Citation: Cao Rundong, Zhu Renhe, Song Juan, Xia Dong, Song Qinqin, Xia Zhiqiang, Liu Mi, Du Haijun, Han Jun, Gao Chen. Rhinovirus infection among severe acute respiratory infection cases in hospitalized children in Xuzhou city, Jiangsu province, 2015−2021[J]. Disease Surveillance, 2023, 38(10): 1205-1210. doi: 10.3784/jbjc.202305220245

2015-2021年江苏省徐州市住院儿童严重急性呼吸道感染病例鼻病毒感染分析

doi: 10.3784/jbjc.202305220245
基金项目: 传染病预防控制国家重点实验室发展基金(No. 2011SKLID104);国家重点研发计划(No. 2022YFC2602202,No. 2022YFC2602402)
详细信息
    作者简介:

    曹润冬,男,江苏省徐州市人,硕士研究生,主要从事呼吸道病毒研究,Email:caord@ivdc.chinacdc.cn

    通讯作者:

    韩俊,Tel:13381210388,Email:hanjun_sci@163.com

    高晨,Tel:13552233803,Email:gaochen@ivdc.chinacdc.cn

  • 中图分类号: R211; R373

Rhinovirus infection among severe acute respiratory infection cases in hospitalized children in Xuzhou city, Jiangsu province, 2015−2021

Funds: This study was supported by the State Key Laboratory of Infectious Disease Prevention and Control (No. 2011SKLID104) and National Key Research and Development Program of China (No. 2022YFC2602202, No. 2022YFC2602402)
More Information
  • 摘要:   目的  了解2015—2021年江苏省徐州市住院儿童严重急性呼吸道感染(SARI)病例中病毒谱的构成,进一步探究人鼻病毒(hRV)的分子流行病学特征。   方法  使用实时荧光定量聚合酶链式反应技术(RT-PCR)对咽拭子标本中的常见呼吸道病毒进行检测。 巢式 PCR 法扩增hRV阳性样本的VP4/VP2区基因序列,构建系统发育树,并进行同源性分析。   结果  1663份标本中呼吸道病毒检出阳性641份(38.54%)。 hRV的检出率位居第1位,为13.95%(232/1663)。 2018年hRV的检出率明显较高(28.08%);呈现夏秋高峰的季节性流行特征。 2020年后,hRV在1—6月的检出率明显降低,但7月后检出率迅速回升,且在3~6岁年龄组中明显上升,≥6岁组中明显下降。 hRV的VP4/VP2区序列比对分析发现涉及29种血清型,以hRV-A组为主。   结论  hRV是2015—2021年徐州市住院儿童SARI病例中主要流行的呼吸道病毒,以hRV-A组为主要流行,血清型具有多样性。 hRV的流行在2020年受到了短暂的抑制之后迅速回升,提示需要对hRV感染进行持续监测。
  • 图  1  2015-2021年徐州市严重急性呼吸道感染儿童病例中鼻病毒检出的月份分布

    Figure  1.  Month distribution of human rhinovirus detection in children with severe acute respiratory infection in Xuzhou, 2015−2021

    图  2  2015-2021年徐州市严重急性呼吸道感染儿童病例中鼻病毒检出率分布

    注:A. 鼻病毒的月检出率变化图;B. 防控措施实施前后鼻病毒在不同年龄组内的变化;C. 构成比分布

    Figure  2.  Distribution of human rhinovirus detection rates in children with severe acute respiratory infection in Xuzhou, 2015-2021

    图  3  基于VP4/VP2区域构建的鼻病毒系统发育进化树

    注:红色标记的菌株为本实验标本株

    Figure  3.  Phylogenetic tree of rhinovirus based on VP4/VP2 region

    表  1  2015-2021年徐州市严重急性呼吸道感染儿童病例中呼吸道病毒的检出情况(n=1663)

    Table  1.   Detection of respiratory virus in children with severe acute respiratory infection in Xuzhou, 2015−2021 (n=1663)

     病毒种类检出数(例)检出率(%)
    鼻病毒23213.95
    呼吸道合胞病毒21813.11
    副流感病毒3型1217.28
    冠状病毒-OC43362.16
    冠状病毒-229E201.20
    甲型流感病毒402.41
    乙型流感病毒130.78
    腺病毒482.89
    下载: 导出CSV

    表  2  2015-2021年徐州市严重急性呼吸道感染儿童病例中人鼻病毒在性别、年龄、季节和年份中的分布情况

    Table  2.   Distribution of human rhinovirus by sex, age, season and year in children with severe acute respiratory infection in Xuzhou city, 2015-2021

    变量病例数鼻病毒χ2P
    检出数
    (例)
    检出率
    (%)
    性别0.327 0.567
     男性104014914.33
     女性6238313.32
    年龄组(岁)4.621 0.202
     <198212512.73
     1~3265717.48
     3~2333314.16
     ≥61221713.93
    季节34.009<0.001
     春3945513.96
     夏4297717.95
     秋4267617.84
     冬414245.80
    年份66.813<0.001
     2015184179.24
     2016290227.59
     20172594115.83
     20182607328.08
     2019270217.78
     20201101917.27
     20212903913.45
    下载: 导出CSV

    表  3  2019-2021年徐州市严重急性呼吸道感染儿童病例中鼻病毒阳性标本的VP4/VP2区基因序列分型结果型结果

    Table  3.   Genotype typing results of VP4/VP2 region of human rhinovirus positive specimens in children with severe acute respiratory infection in Xuzhou, 2019−2021

    种群亚型数量/条 种群型别数量/条
    hRV-A A2 1 hRV-A A85 1
    hRV-A A7 1 hRV-A A89 1
    hRV-A A8 1 hRV-A A105 2
    hRV-A A15 1 hRV-A A106 1
    hRV-A A19 1 hRV-B B3 1
    hRV-A A20 1 hRV-B B42 1
    hRV-A A29 1 hRV-B B48 1
    hRV-A A30 1 hRV-B B70 1
    hRV-A A38 1 hRV-C C5 1
    hRV-A A45 1 hRV-C C8 2
    hRV-A A49 5 hRV-C C19 1
    hRV-A A56 1 hRV-C C23 2
    hRV-A A58 1 hRV-C C40 1
    hRV-A A78 1 hRV-C C45 1
    hRV-A A80 1
    下载: 导出CSV
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  • 收稿日期:  2023-05-22
  • 录用日期:  2023-10-09
  • 网络出版日期:  2023-10-09
  • 刊出日期:  2023-11-07

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