2019－2021年新疆维吾尔自治区蜱与人感染塔城蜱病毒5监测分析

李逢时; 赵姗姗; 谭文波; 吾热力哈孜·哈孜汗; 谷新利; 王素文; 刘钢; 王远志

doi:10.3784/jbjc.202305310258

2019－2021年新疆维吾尔自治区蜱与人感染塔城蜱病毒5监测分析

doi: 10.3784/jbjc.202305310258

1.
石河子大学医学院, 新疆石河子 832003
2.
石河子大学动物科技学院, 新疆石河子 832003

基金项目: 国家重点研发计划项目（No. 2022YFC2304000）；国家自然科学基金项目（No. 82260399）；兵团重点领域科技攻关计划项目（No. 2022AB014）

详细信息

作者简介:
李逢时，女，山东省济南市人，在读硕士研究生，主要从事病原生物学研究，Email：857255674@qq.com

通讯作者:
王远志，Tel：15886931218，Email：wangyuanzhi621@ 126.com

中图分类号: R211；R373
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出版历程
- 收稿日期: 2023-05-31
- 网络出版日期: 2023-09-19

Surveillance for tick and humans infection with Tacheng tick virus 5 in Xinjiang Uygur Autonomous Region, 2019−2021

1.
School of Medicine, Shihezi University, Shihezi 832003, Xinjiang, China
2.
College of Animal Science & Tecnology, Shihezi University, Shihezi 832003, Xinjiang, China

Funds: This study was supported by the fund for National Key Research and Development Plan Project (No. 2022YFC2304000), National Natural Science Foundation of China (No. 82260399) and the fund for Scientific and Technological Projects in Key Areas of the Corps (No. 2022AB014)

More Information

Corresponding author: Wang Yuanzhi, Email: wangyuanzhi621@126.com

摘要

摘要: 目的对塔城蜱病毒5（Tacheng Tick Virus 5, TcTV-5）进行检测，为新发蜱媒传染病的监测提供参考。方法 2019 — 2021年采集新疆维吾尔自治区（新疆）6县（市）的寄生蜱和游离蜱451只，对其分别进行形态学和分子生物学鉴定；于2021年收集新疆图木舒克市农牧民血液样本280份，运用反转录聚合酶链式反应（RT-PCR）技术，对收集到的所有样本进行TcTV-5感染阳性率的检测，构建基因进化树，分析遗传进化关系，对检测结果进行分子流行病学分析。结果经形态和分子鉴定，在新疆采集到的蜱分属于3属3种，包括璃眼蜱属的亚洲璃眼蜱、革蜱属的边缘革蜱和扇头蜱属的图兰扇头蜱；新疆蜱与人中均可检测到TcTV-5，6个县（市）的蜱阳性率为3.33% （2/60）～36.11% （26/72），图木舒克市280份人血样本的阳性率为2.50%（7/280）。结论 TcTV-5在新疆蜱与人血中均可被检出；在多种蜱种（亚洲璃眼蜱、边缘革蜱、图兰扇头蜱）中均可检测到，边缘革蜱阳性率最高。
- 塔城蜱病毒5 /
- 蜱 /
- 新疆维吾尔自治区 /
- 蜱传病
Abstract: Objective To detect Tacheng tick virus 5 (TcTV-5) in ticks and humans in Xinjiang Uygur Autonomous Region（Xinjiang), and provide reference for the detection of new tick-borne infectious diseases. Methods A total of 451 free ticks and parasitic ticks were collected from 6 counties in Xinjiang from 2019 to 2021. Morphological and molecular biological tests were performed to determine tick species. In 2021, a total of 280 blood samples of farmers and herdsmen in Tumushuke of Xinjiang were collected, Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect TcTV-5 in collected samples, then gene phylogenetic tree was constructed, the genetic evolutionary relationship was analyzed, and the results were analyzed by molecular epidemiological method. Results According to morphological and molecular identifications, the ticks collected in Xinjiang were classified into three genera and three species, including Hyalomma asiaticum, Dermacentor marginatus and Rhipicephalus turanicus. TcTV-5 was detected in both ticks and human blood samples.The positive rates of TcTV-5 in ticks in 6 counties ranged from 3.33% (2/60)–36.11% (26/72), and the positive rates of TcTV-5 was 2.50% (7/280) in 280 human blood samples from Tumushuke. Conclusion TcTV-5 can be detected in both ticks and human blood in Xinjiang. The ticks carrying TcTV-5 included (H. asiaticum, D. marginatus and R. turanicus)and the positive rate was highest in D. marginatus.
- Tacheng tick virus 5 /
- Tick /
- Xinjiang Uygur Autonomous Region /
- Tick-borne disease

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图 1 人和蜱的塔城蜱病毒 5的L基因进化树

注：●. 蜱中携带的塔城蜱病毒5基因； ■. 人血中携带的塔城蜱病毒5基因； Changping Tick Virus 3.昌平蜱病毒3； Genoa Virus.热那亚病毒；Lonestar Tick Chuvirus 1.孤星蜱楚病毒1；Wuhan Tick Virus 2.武汉蜱病毒2； Bole Tick Virus 3.博乐蜱病毒3； Changping Tick Virus 2.昌平蜱病毒2；Karukera Tick Virus.卡鲁凯拉蜱病毒；Wuhan Mosquito Virus 8.武汉蚊病毒8； Blacklegged Tick Chuvirus 2.黑腿硬蜱楚病毒2； Hubei Chuvirus-like Virus 3.湖北类楚病毒3； Lampyris Noctiluca Chuvirus-lie Virus 1.大萤火虫类楚病毒1； Wuchang Cockraoch Virus 3.武昌蟑螂病毒3； Lishi Spider Virus 1.李氏蜘蛛病毒1； Hubei Chuvirus-like Virus 1.湖北类楚病毒1； Tacheng Tick Virus 4.塔城蜱病毒4

Figure 1. Phylogenetic tree of L gene of Tacheng tick virus 5 from humans and ticks

下载: 全尺寸图片幻灯片

表 1 2019－2021年新疆维吾尔自治区蜱采集详细信息

Table 1. Detailed information of tick collection in Xinjiang Uygur Autonomous Region, 2019−2021

采集地点采集年份	寄生蜱（只）	游离蜱（只）	寄生宿主
阿拉山口市 2020	72	/	牛
玛纳斯县 2021	72	36	羊
石河子市 2021	60	/	骆驼
巴楚县 2019	/	85	/
温泉县 2021	/	60	/
塔城市 2021	60	/	牛
合计	264	181
注：/ . 未采集到蜱或无寄生宿主

下载: 导出CSV

表 2 塔城蜱病毒5引物序列信息

Table 2. Primer sequence information of Tacheng Tick Virus 5

病毒	引物名称	扩增片段	引物序列（5' ～3'）	区间位点（bp）	片段大小（bp）
Tacheng Tick Virus 5	TC5-F1	L	TGGTCAGATGGGAGGAAT	2 105	605
	TC5-F2		TATGGTTCCTCCAAAAGC	2 243	467
	TC5-R1		CCGCCCTAGCAAAGAAAT	2 709

下载: 导出CSV

表 3 新疆维吾尔自治区蜱携带塔城蜱病毒 5的阳性率

Table 3. Positive rate of Tacheng Tick Virus 5 carried by ticks in Xinjiang Uygur Autonomous Region

地区	蜱种鉴定结果	阳性率（%）	（a/b）
阿拉山口市	边缘革蜱	36.11	（26/72）
玛纳斯县	亚洲璃眼蜱	寄生：18.06	（13/72）
		游离：0.00	（0/36）
石河子市	图兰扇头蜱	3.33	（2/60）
巴楚县	亚洲璃眼蜱	3.53	（3/85）
温泉县	边缘革蜱	11.67	（7/60）
塔城市	边缘革蜱	26.67	（16/60）
注：a. 阳性样本总数；b. 实验样本总数

下载: 导出CSV

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