Abstract:
Objective To evaluate the anti-asthma function of Bacteroides caccae IM01 and its effect on the gut microbiota using the optimized mouse model of allergic asthma.
Methods An ovalbumin (OVA)-induced asthmatic mouse model was optimized by comparing different challenge protocols. Female BALB/c mice were randomly divided into phosphate-buffered saline control group, OVA model group, and IM01 intervention group. The IM01 group received daily oral administration of 1×109 colony-forming unit/mouse live IM01 for 21 days. Lung tissues of the mice were collected on day 22 for the detections of serum OVA-specific immunoglobulin E (IgE) level, lung Interleukin (IL)-4, IL-5, and IL-13 concentrations, and H&E staining of lung histopathology and 16S rRNA sequencing of fecal microbiota were conducted.
Results The OVA aerosol challenge protocol demonstrated superior model establishment. Oral administration of IM01 significantly reduced serum IgE and lung IL-4/IL-5/IL-13 levels in asthmatic mice, while alleviating pathological inflammatory cell infiltration; IM01 modulated gut microbiota composition (increasing beneficial bacteria and decreasing pathogenic bacteria).
Conclusion Oral administration of IM01 alleviates lung inflammation and pathological damage in asthmatic mice while regulating gut microbiota profiles
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