Abstract:
Objective To evaluate the quality of antimicrobial susceptibility testing (AST) for Enterobacteriaceae in laboratories in China Pathogen Identification Network (China PIN) from 2021 to 2024 and provide data support for the improvement of of surveillance quality.
Methods The national central laboratory distributed 6 test strains (Enterobacteriaceae) to provincial central laboratories and prefectural (municipal) laboratories in the network from 2021 to 2024. Each network laboratory conducted AST to 17 types of antibiotics. The testing results were reported through China PIN information system. The AST was conducted based on the surveillance technical manual. Software R 4.3.2 was used to analyze the results from laboratories in different regions in China.
Results From 2021 to 2024, the accuracy rate of minimum inhibitory concentration test of the six Enterobacteriaceae species was 90.49%, and the accuracy rate of sensitivity/intermediate/resistance was 97.81%. Of the deviation values, 89.36% were distributed within the 1 gradient of reference value. In addition to the reference value, the results of amikacin susceptibility test were all distributed above 1 gradient of reference value. Azithromycin susceptibility test results showed the widest gradient range from <5 gradients to >5 gradients of the reference value. Statistical analysis revealed significant regional differences (P<0.05) in AST accuracy for QC2022_01, QC2024_02, and six Enterobacteriaceae species. The accuracy rate of AST for 6 species in the network laboratories of northern China was highest.
Conclusion To improve the accuracy of AST in the laboratory network, it is necessary to strengthen the quality control and promote standardized test methods. Differences existed in the AST performance among the laboratories in different regions, it is important to improve training and technical guidance in network laboratories in northeastern, northwestern and southwestern China.
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