2017-2020年湖北省甲型H3N2型流感病毒流行特征和进化分析

谢蒙生 方斌 黄成静 于甜甜 韩诗 陈丹

谢蒙生, 方斌, 黄成静, 于甜甜, 韩诗, 陈丹. 2017-2020年湖北省甲型H3N2型流感病毒流行特征和进化分析[J]. 疾病监测. doi: 10.3784/jbjc.
引用本文: 谢蒙生, 方斌, 黄成静, 于甜甜, 韩诗, 陈丹. 2017-2020年湖北省甲型H3N2型流感病毒流行特征和进化分析[J]. 疾病监测. doi: 10.3784/jbjc.
Xie Mengsheng, Fang Bin, Huang Chengjing, Yu Tiantian, Han Shi, Chen Dan. Epidemiological and evolutionary analysis on influenza A (H3N2) virus in Hubei, 2017–2020[J]. Disease Surveillance. doi: 10.3784/jbjc.
Citation: Xie Mengsheng, Fang Bin, Huang Chengjing, Yu Tiantian, Han Shi, Chen Dan. Epidemiological and evolutionary analysis on influenza A (H3N2) virus in Hubei, 2017–2020[J]. Disease Surveillance. doi: 10.3784/jbjc.

2017-2020年湖北省甲型H3N2型流感病毒流行特征和进化分析

doi: 10.3784/jbjc.
基金项目: 国家科技重大专项(No.2018ZX10734-401)
详细信息
    作者简介:

    谢蒙生,男,湖北省恩施市人,硕士,主要从事流感和禽流感病毒相关研究,Email:Deogratias1@163.com

    通讯作者:

    方斌,Tel:027-87740787,Email:nicolfang@163.com

    陈丹,Tel:027-6889-3436,Email:chendan@wust.edu.cn

Epidemiological and evolutionary analysis on influenza A (H3N2) virus in Hubei, 2017–2020

Funds: This study was supported by the National Science and Technology Major Project (No.2018ZX10734-401)
More Information
  • 摘要:   目的  了解2017 — 2020年湖北省甲型H3N2型流感流行分布及基因进化情况。  方法  根据湖北省流感病原学监测数据,分析2017 — 2020年甲型H3N2型流感病毒流行分布情况,按年度分布和地域分布的原则选取2017 — 2020年各市级流感监测网络实验室送检的该亚型流感病毒共38株进行测序,获得病毒HA和NA氨基酸序列,分析其抗原决定簇氨基酸位点变化情况,对其进行基因进化和三维建模分析。  结果  2017 — 2020年湖北省甲型H3N2型流感病毒存在3个流行高峰,第Ⅰ流行峰主要流行3C.2a1簇病毒,第Ⅱ流行峰主要为3C.2a1b+T131K簇病毒,第Ⅲ流行峰主要为3C.2a1b+T135K簇病毒。3C.2a1b+T131K簇病毒与3C.2a1b+T135K簇病毒在HA蛋白3处抗原决定簇上存在8处不同氨基酸突变位点。3C.2a1b+T131K簇病毒的50、131、135位140位点和3C.2a1b+T135K簇病毒在三维模拟结构图中差异明显。  结论  2017 — 2020年湖北省甲型H3N2型流感病毒不断进化,加强对近期3C.2a1b+T135K簇病毒的进化监测和抗原突变分析,有助于提高湖北省流感病毒流行病学和基因进化监测水平。
  • 图  1  2017-2020年湖北省甲型H3N2型流感病毒核酸检测阳性率周分布

    Figure  1.  Weekly distribution of nucleic acid positive rate of influenza A(H3N2)virus in Hubei, 2017–2020

    图  2  2017-2020年湖北省甲型H3N2型流感病毒基因进化树

    注:A. HA基因;B. NA基因;NA.神经氨酸酶;HA. 血凝素;紫色、蓝色、绿色、橙色毒株名分别对应2017—2020年湖北省甲型流感毒株,红色为参考代表株,参考代表株后采用Clade 3C.3a注释该毒株的进化分支号;S245N等为进化簇氨基酸突变位点(A);S91N等为进化簇氨基酸突变位点(B);0.004等数值为进化距离

    Figure  2.  Phylogenetic tree of genes of influenza A(H3N2)virus in Hubei, 2017–2020

    图  3  湖北省H3N2亚型流感病毒HA单体模拟图

    注:侧方图A为逆时针旋转90°,侧方图B为顺时针旋转90°

    Figure  3.  Simulation diagram of HA monomer of H3N2 subtype influenza virus in Hubei Province

    表  1  2017-2020年湖北省甲型H3N2型流感病毒流行高峰期核酸阳性率

    Table  1.   Nucleic acid positive rate of influenza A(H3N2)virus during detection peak periods in Hubei, 2017–2020

    流行高峰期流行高峰周平均阳性率(%)核酸阳性率峰值(%)
    2017年第29周至
    2017年第38周
    23.9835.43
    2019年第3周至
    2019年第13周
    5.01 9.03
    2019年第48周至
    2020年第4周
    33.8751.36
    下载: 导出CSV

    表  2  2017-2020年湖北省甲型H3N2型流感病毒HA蛋白抗原表位氨基酸突变位点和样本信息

    Table  2.   Amino acid mutation site and sample information of HA protein epitope of influenza A(H3N2)virus in Hubei, 2017–2020

    毒株名采集周次基因簇流行高峰抗原决定簇A抗原决定簇B
    111111111 11111111
    233334445 25689999
    615780240 89060378
    A/Switzerland/9715293/2013NTTSSIGNRASKGDFQS
    A/hubei-wujiagang/19/202020/013c.2a1b+T135K..K....S..YTDNS.P
    A/hubei-dongbao/32/202020/013c.2a1b+T135KS.KF.R.S..YT..S..
    A/hubei-chongyang/32/202020/013c.2a1b+T135KS.KF.R.S..YT..S..
    A/hubei-tongcheng/36/201919/523c.2a1b+T135KS.KF.R.S..YT..S..
    A/hubei-hongshan/312/201919/513c.2a1b+T135K..KF...S..YT..S..
    A/hubei-zhijiang/53/201919/513c.2a1b+T135K..KF...S..YT..S..
    A/hubei-xiangcheng/1936/201919/473c.2a1b+T135K..KF...S..Y...S..
    A/hubei-fancheng/1949/201919/463c.2a1b+T135K..KF...S..YT..S..
    A/hubei-xiling/1766/201919/443c.2a1b+T135K..KF...S..YT..S..
    A/hubei-xiling/1757/201919/443c.2a1b+T135K..KF...S..YT..S..
    A/hubei-xiling/1742/201919/443c.2a1b+T135K..KF...S..YT..S..
    A/hubei-wujiagang/1361/201919/183c.2a1b+T135K..KF...S..YT..S..
    A/hubei-maojian/1406/201919/073c.2a1b+T135K..KF...S..YT..S..
    A/hubei-xiling/1276/201818/143c.2a1b+T135K..K.A..S..YT.....
    A/hubei-maojian/12089/201919/473c.2a1b+T131K.K..A..S.TYT.....
    A/hubei-xiling/1178/201919/093c.2a1b+T131K.K..A..S.TYT...R.
    A/hubei-jiangan/1133/201919/073c.2a1b+T131K.K..A..S.TYT.....
    A/hubei-jiangan/176/201919/043c.2a1b+T131K.K..A..S.TYT..S..
    A/hubei-jiangan/132/201919/023c.2a1b+T131K.K..A..S.TYT..S..
    A/hubei-maojian/1153/201818/033c.2a2.K..A.KS.TYT.....
    A/hubei-enshi/1887/201717/433c.2a2.K..A.KS.TY......
    A/hubei-huangshigang/1894/201717/413c.2a2.K..A.KS.TY......
    A/hubei-xiling/1428/201717/373c.2a2.K..A.KS.TYT.....
    A/hubei-jiangan/1618/201717/313c.2a2.K..A.KS.TYT.....
    A/hubei-wujiagang/1144/201717/073c.2a2.K..A.KS.TY......
    A/hubei-huangzhou/118/201717/013c.2a2.K..A.KS.TYA.....
    A/hubei-lichuan/510/201919/143c.2a1..K.A..S.TYT.....
    A/hubei-qianjiang/2143/201717/373c.2a1..N.A.RS.TYT.....
    A/hubei-jiangan/1640/201717/323c.2a1..N.A.RS.TYT.....
    A/hubei-jiangan/1636/201717/323c.2a1..N.A.RS.TYT.....
    A/hubei-jiangan/1578/201717/293c.2a1..N.A.RS.TY......
    A/hubei-xianan/172/201717/043c.2a1..K.A.KS.TYT.....
    A/hubei-enshi/33/201919/093c2a3..K.A.RKKTYT.....
    A/hubei-xianfeng/38/201919/023c2a3..K.A.RKKTYT.....
    A/hubei-hefeng/51/201717/493c2a3..K.A.RKKTYT.....
    A/hubei-dongbao/1618/201717/313c2a3..K.A.RKKTYT.....
    A/hubei-jiangan/1619/201717/313c2a3..K.A..KKTYT.....
    A/hubei-xianan/16/201919/013c3a.......K......S..
    下载: 导出CSV
    续表 2
    毒株名采集周次基因簇流行高峰抗原决定簇C抗原决定簇D抗原决定簇E
    000233112200002
    455711271167996
    704912113928121
    A/Switzerland/9715293/2013SESSQSNNVSEGSKR
    A/hubei-wujiagang/19/202020/013c.2a1b+T135K......KK..G..R.
    A/hubei-dongbao/32/202020/013c.2a1b+T135K.K....KK..G..R.
    A/hubei-chongyang/32/202020/013c.2a1b+T135K.K....KK..G..R.
    A/hubei-tongcheng/36/201919/523c.2a1b+T135K.K....KK..G..R.
    A/hubei-hongshan/312/201919/513c.2a1b+T135K......KK..G..R.
    A/hubei-zhijiang/53/201919/513c.2a1b+T135K......KK..G..R.
    A/hubei-xiangcheng/1936/201919/473c.2a1b+T135K......KK..G..R.
    A/hubei-fancheng/1949/201919/463c.2a1b+T135K......KK..G..R.
    A/hubei-xiling/1766/201919/443c.2a1b+T135K......KK..G..R.
    A/hubei-xiling/1757/201919/443c.2a1b+T135K......KK..G..R.
    A/hubei-xiling/1742/201919/443c.2a1b+T135K......KK..G..R.
    A/hubei-wujiagang/1361/201919/183c.2a1b+T135K......KK..G..R.
    A/hubei-maojian/1406/201919/073c.2a1b+T135K......KK..G..R.
    A/hubei-xiling/1276/201818/143c.2a1b+T135K......KKA.G..R.
    A/hubei-maojian/12089/201919/473c.2a1b+T131K..R..NKK.YG..R.
    A/hubei-xiling/1178/201919/093c.2a1b+T131KP.....KK.FG..R.
    A/hubei-jiangan/1133/201919/073c.2a1b+T131K......KK.FGS.R.
    A/hubei-jiangan/176/201919/043c.2a1b+T131K......KK..G..R.
    A/hubei-jiangan/132/201919/023c.2a1b+T131K......KK..G..R.
    A/hubei-maojian/1153/201818/033c.2a2....H.........Q
    A/hubei-enshi/1887/201717/433c.2a2....H.........Q
    A/hubei-huangshigang/1894/201717/413c.2a2....H.........Q
    A/hubei-xiling/1428/201717/373c.2a2....H.........Q
    A/hubei-jiangan/1618/201717/313c.2a2....H.........Q
    A/hubei-wujiagang/1144/201717/073c.2a2....H.........Q
    A/hubei-huangzhou/118/201717/013c.2a2...YH.........Q
    A/hubei-lichuan/510/201919/143c.2a1......KK......Q
    A/hubei-qianjiang/2143/201717/373c.2a1......KK.....R.
    A/hubei-jiangan/1640/201717/323c.2a1......KK.....R.
    A/hubei-jiangan/1636/201717/323c.2a1......KK.....R.
    A/hubei-jiangan/1578/201717/293c.2a1......KK.....R.
    A/hubei-xianan/172/201717/043c.2a1....H..K.......
    A/hubei-enshi/33/201919/093c2a3....H.K.......Q
    A/hubei-xianfeng/38/201919/023c2a3....H.K.......Q
    A/hubei-hefeng/51/201717/493c2a3....H.K.......Q
    A/hubei-dongbao/1618/201717/313c2a3....H.K.......Q
    A/hubei-jiangan/1619/201717/313c2a3....H.K.......Q
    A/hubei-xianan/16/201919/013c3a............N..
    注:-. 未在流行高峰期内;·为未发生变异
    下载: 导出CSV
  • [1] 卢亦愚. 流感病毒的变异及其引发流行的预防[J]. 国外医学流行病学-传染病学分册,2005,32(5):257–259.

    Lu YY. The prevention of the epidemic of influenza caused by varied influenza virus[J]. For Med Sci Epidemiol Lemol, 2005, 32(5): 257–259.
    [2] Cheung TKW, Poon LLM. Biology of influenza a virus[J]. Ann N Y Acad Sci, 2007, 1102(1): 1–25. DOI:  10.1196/annals.1408.001.
    [3] Rambaut A, Pybus OG, Nelson MI, et al. The genomic and epidemiological dynamics of human influenza A virus[J]. Nature, 2008, 453(7195): 615–619. DOI:  10.1038/nature06945.
    [4] 李文娟, 王大燕. 我国流感疾病负担相关研究进展[J]. 中国人兽共患病学报,2019,35(10):928–933. DOI:10.3969/j.issn.1002−2694.2019.00.110.

    Li WJ, Wang DY. Research progress on the disease burden of influenza in China[J]. Chin J Zoon, 2019, 35(10): 928–933. DOI: 10.3969/j.issn.1002−2694.2019.00.110.
    [5] 杜雪飞, 丁洁, 乔梦凯, 等. 南京市2007-2013年H3N2流感HA分子进化特征分析[J]. 中国人兽共患病学报,2015,31(9):845–849. DOI:10.3969/j.issn.1002−2694.2015.09.013.

    Du XF, Ding J, Qiao MK, et al. Molecular evolution characteristics of HA gene of H3N2 human influenza A viruses between 2007 and 2013 in Nanjing, Jiangsu province, China[J]. Chin J Zoon, 2015, 31(9): 845–849. DOI: 10.3969/j.issn.1002−2694.2015.09.013.
    [6] 李向忠, 方芳, 陈则. 流感病毒神经氨酸酶不同区域的作用[J]. 生命科学研究,2005,9(2 Suppl 1):59–65.

    Li XZ, Fang F, Chen Z, et al. Roles of different domains of influenza virus neuraminidase[J]. Life Sci Res, 2005, 9(2 Suppl 1): 59–65.
    [7] 熊成龙, 张志杰, 梁笛, 等. 甲型流感病毒血凝素基因的进化与分型研究[J]. 复旦学报(医学版),2011,38(4):46–50. DOI:10.3969/j.issn.1672−8467.2011.04.009.

    Xiong CL, Zhang ZJ, Liang D, et al. Evolution and classification of haemagglutinin genes of influenza A[J]. Fudan Univ J Med Sci, 2011, 38(4): 46–50. DOI: 10.3969/j.issn.1672−8467.2011.04.009.
    [8] Daniels R, Ermetal B, Rattigan A, et al. Influenza virus characterisation, summary Europe, March 2020[R]. Stockholm: ECDC, 2021.
    [9] Skowronski DM, Janjua NZ, Serres GD, et al. Low 2012-13 influenza vaccine effectiveness associated with mutation in the egg-adapted H3N2 vaccine strain not antigenic drift in circulating viruses[J]. PLoS One, 2014, 9(3): e92153. DOI:  10.1371/journal.pone.0092153.
    [10] 仝振东, 蒲柳艳, 虞奇跃, 等. 一种基于流感哨点监测的流感预警分析方法[J]. 疾病监测,2011,26(5):386–387, 391. DOI:10.3784/j.issn.1003−9961.2011.05.016.

    Tong ZD, Pu LY, Yu QY, et al. Early warning of influenza epidemic based on influenza sentinel surveillance[J]. Dis Surveill, 2011, 26(5): 386–387, 391. DOI: 10.3784/j.issn.1003−9961.2011.05.016.
    [11] Cowling BJ, Wong IOL, Ho LM, et al. Methods for monitoring influenza surveillance data[J]. Int J Epidemiol, 2006, 35(5): 1314–1321. DOI:  10.1093/ije/dyl162.
    [12] Yen HL, Hoffmann E, Taylor G, et al. Importance of neuraminidase active-site residues to the neuraminidase inhibitor resistance of influenza viruses[J]. J Virol, 2006, 80(17): 8787–8795. DOI: 10.1128/JVI.00477−06.
    [13] Arnold K, Bordoli L, Kopp J, et al. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling[J]. Bioinformatics, 2006, 22(2): 195–201. DOI:  10.1093/bioinformatics/bti770.
    [14] 刘琳琳, 韩诗, 余晓, 等. 2016-2019年湖北省流感疫情及病原学监测分析[J]. 疾病监测,2020,35(12):1105–1109. DOI:10.3784/j.issn.1003−9961.2020.12.010.

    Liu LL, Han S, Yu X, et al. Epidemiology and etiology of influenza in Hubei province, 2016-2019[J]. Dis Surveill, 2020, 35(12): 1105–1109. DOI: 10.3784/j.issn.1003−9961.2020.12.010.
    [15] Hua S, Li XY, Liu M, et al. Antigenic variation of the human influenza A (H3N2) virus during the 2014-2015 winter season[J]. Sci China Life Sci, 2015, 58(9): 882–888. DOI: 10.1007/s11427−015−4899−z.
    [16] Li J, Zhou YY, Kou Y, et al. Interim estimates of divergence date and vaccine strain match of human influenza A(H3N2) virus from systematic influenza surveillance (2010-2015) in Hangzhou, southeast of China[J]. Int J Infect Dis, 2015, 40: 17–24. DOI:  10.1016/j.ijid.2015.09.013.
    [17] Bedford T, Suchard MA, Lemey P, et al. Integrating influenza antigenic dynamics with molecular evolution[J]. Elife, 2014, 3(4): e01914. DOI:  10.7554/eLife.01914.
    [18] 张晶波, 张家淮, 胡晓芬, 等. 北京市西城区2009年-2011年H3N2甲型流感病毒HA1基因特性分析[J]. 中国卫生检验杂志,2013,23(3):661–663.

    Zhang JB, Zhang JH, Hu XF, et al. Gene analysis of HA1 of A influenza virus H3N2 in Xicheng district, Beijing[J]. Chin J Health Lab Technol, 2013, 23(3): 661–663.
    [19] Skehel JJ, Wiley DC. Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin[J]. Annu Rev Biochem, 2000, 69: 531–569. DOI:  10.1146/annurev.biochem.69.1.531.
    [20] Koel BF, Burke DF, Bestebroer TM, et al. Substitutions near the receptor binding site determine major antigenic change during influenza virus evolution[J]. Science, 2013, 342(6161): 976–979. DOI:  10.1126/science.1244730.
    [21] 李希妍, 成艳辉, 谭敏菊, 等. 2013-2014年度中国H3N2亚型流感病毒病原学特征分析[J]. 病毒学报,2015,31(1):30–35. DOI: 10.13242/j.cnki.bingduxuebao.002622.

    Li XY, Chen YH, Tang MJ, et al. Virological characteristics of influenza A (H3N2) virus in mainland China during 2013-2014[J]. Chin J Virol, 2015, 31(1): 30–35. DOI:  10.13242/j.cnki.bingduxuebao.002622.
    [22] Sun SS, Wang QZ, Zhao F, et al. Prediction of biological functions on glycosylation site migrations in human influenza H1N1 viruses[J]. PLoS One, 2012, 7(2): e32119. DOI:  10.1371/journal.pone.0032119.
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