一起疑似食物中毒暴发事件的病原学分析

李娜 关恒云 时玉雯 刘岚铮 姜慧钰 刘铭 尹晓煜 陈潇 宋绚丽 刘辉

李娜, 关恒云, 时玉雯, 刘岚铮, 姜慧钰, 刘铭, 尹晓煜, 陈潇, 宋绚丽, 刘辉. 一起疑似食物中毒暴发事件的病原学分析[J]. 疾病监测.
引用本文: 李娜, 关恒云, 时玉雯, 刘岚铮, 姜慧钰, 刘铭, 尹晓煜, 陈潇, 宋绚丽, 刘辉. 一起疑似食物中毒暴发事件的病原学分析[J]. 疾病监测.
Li Na, Guan Hengyun, Shi Yuwen, Liu Lanzheng, Jiang Huiyu, Liu Ming, Yin Xiaoyu, Chen Xiao, Song Xuanli, Liu Hui. Etiological analysis on an outbreak of suspected food poisoning[J]. Disease Surveillance.
Citation: Li Na, Guan Hengyun, Shi Yuwen, Liu Lanzheng, Jiang Huiyu, Liu Ming, Yin Xiaoyu, Chen Xiao, Song Xuanli, Liu Hui. Etiological analysis on an outbreak of suspected food poisoning[J]. Disease Surveillance.

一起疑似食物中毒暴发事件的病原学分析

基金项目: 济南市卫生健康委员会科技计划项目(No.2020-4-112,No.2020-4-113)
详细信息
    作者简介:

    李娜,女,山东省滨州市人,硕士研究生,主要从事分子生物学与分子免疫学工作,Email:1129910161@qq.com

    关恒云,女,山东省济南市人,硕士研究生,主要从事微生物分子流行病学研究,Email:guanhengyun@126.com

    通讯作者:

    刘辉,Tel:0531-81278920,Email:dahuiliu1981@sina.com

  • 中图分类号: R211;R155.5

Etiological analysis on an outbreak of suspected food poisoning

Funds: This study was supported by the Science and Technology Project of Jinan Municipal Health Commission (No.2020-4-112, No.2020-4-113)
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  • 摘要:   目的  阐明一起疑似由蜡样芽胞杆菌污染奶源引起的食物中毒暴发事件的病原学特征,为查明暴发源头和临床治疗提供依据。  方法  对198例患儿进行流行病学调查,采集可疑奶制品和病例标本,进行菌株分离和鉴定。分离到的蜡样芽胞杆菌菌株进行脉冲场凝胶电泳(PFGE)分子分型、全基因组测序以及毒力基因分析。采用微量肉汤稀释法进行药物敏感性测定。  结果  流行病学调查显示198例患儿均有不同程度的腹痛、腹泻和发热等症状,符合食源性疾病暴发定义。共分离到25株蜡样芽孢杆菌(5株来自奶制品,20株分离自病例标本)。PFGE分型分析显示,13株病例标本菌株与5株奶源性菌株条带具有高度相似性。25株蜡样芽孢杆菌对环丙沙星、万古霉素、四环素、氯霉素、庆大霉素和亚胺培南均敏感,但对氨苄西林、青霉素完全耐药。  结论  该起食物中毒暴发事件疑似由蜡样芽胞杆菌污染引起,应加大食品安全知识的宣传普及,提高食品安全意识,预防类似事件的发生。
  • 图  1  25株蜡样芽胞杆菌的脉冲场凝胶电泳图谱聚类结果

    Figure  1.  PFGE subtyping of 25 Bacillus cereus strains

    表  1  济南市12家幼儿园疑似病例数及罹患率

    Table  1.   Number of suspected cases and attack rate in 12 kindergartens in Jinan

    区县出现病例的幼儿园数(家)幼儿园编号饮用奶制品人数疑似病例数(例)罹患率(%)
    天桥区6 1 126 3930.95
    2 79 2835.44
    3 56 916.07
    4 46 4 8.70
    5 30 2 6.67
    6 25 1 4.00
    合计 362 8322.93
    槐荫区4 7 114 1714.91
    8 230 15 6.52
    9 88 8 9.09
    10 71 2 2.82
    合计 500 42 8.40
    市中区111 197 5829.44
    历城区112 106 1514.15
    合计1 16519817.00
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