北京市一起由宋内志贺菌引起的细菌性痢疾暴发的病原学分析

吕冰 田祎 杨艳娜 甄博珺 贾蕾 林长缨 张新 黄瑛 曲梅

吕冰, 田祎, 杨艳娜, 甄博珺, 贾蕾, 林长缨, 张新, 黄瑛, 曲梅. 北京市一起由宋内志贺菌引起的细菌性痢疾暴发的病原学分析[J]. 疾病监测.
引用本文: 吕冰, 田祎, 杨艳娜, 甄博珺, 贾蕾, 林长缨, 张新, 黄瑛, 曲梅. 北京市一起由宋内志贺菌引起的细菌性痢疾暴发的病原学分析[J]. 疾病监测.
Lyv Bing, Tian Yi, Yang Yanna, Zhen Bojun, Jia Lei, Lin Changying, Zhang Xin, Huang Ying, Qu Mei. Etiological analysis on a bacillary dysentery outbreak caused by Shigella sonnei in Beijing[J]. Disease Surveillance.
Citation: Lyv Bing, Tian Yi, Yang Yanna, Zhen Bojun, Jia Lei, Lin Changying, Zhang Xin, Huang Ying, Qu Mei. Etiological analysis on a bacillary dysentery outbreak caused by Shigella sonnei in Beijing[J]. Disease Surveillance.

北京市一起由宋内志贺菌引起的细菌性痢疾暴发的病原学分析

基金项目: 国家科技重大专项(No.2018ZX10714002-003-002)
详细信息
    作者简介:

    吕冰,女,黑龙江省哈尔滨市人,研究员,主要从事肠道病原监测工作,Email:lvbing2001@163.com

    通讯作者:

    曲梅,Tel:010-64407115,Email:meiqu@126.com

  • 中图分类号: R211;R378.2

Etiological analysis on a bacillary dysentery outbreak caused by Shigella sonnei in Beijing

Funds: This study was supported by the National Science and Technology Major Project (No.2018ZX10714002-003-002)
More Information
  • 摘要:   目的  对北京市一起由宋内志贺菌引起的暴发疫情进行流行病学调查以及病原学分析,为此类疫情处理提供参考。  方法  2019年9月5 — 20日,采集病例及厨师粪便/肛拭子样本,以及水样、外环境等标本进行病原分离鉴定,并将分离到的病原菌进行药物敏感性试验、脉冲场凝胶电泳(PFGE)分子分型分析、全基因组测序,利用全基因组数据分析病原菌的耐药、毒力相关基因,并进行多位点序列分型(MLST)分析,构建全基因组单核苷酸多态性(wgSNP)分型树状图。  结果  共分离到16株宋内志贺菌和1株肠产毒性大肠埃希菌(ETEC),志贺菌均对氨苄西林、四环素、复方新诺明、头孢唑啉、头孢噻肟、庆大霉素、萘啶酸、阿奇霉素、多西环素耐药,且均为产超广谱β-内酰胺酶(ESBLs)菌株。均为ST152型,是国内常见型别。其中1株宋内志贺菌全基因组中存在insA插入序列,而在其他志贺菌全基因组中未发现,和其同时分离到的ETEC全基因组中发现有该插入序列。  结论  本次疫情是由多耐药宋内志贺菌引起。在病原溯源研究中,全基因组数据分析是PFGE分型的必要补充。
  • 图  1  16株宋内志贺菌的脉冲场凝胶电泳图谱聚类结果

    注:AMP.氨苄西林;AMC.阿莫西林-克拉维酸钾;CFZ.头孢唑啉;FEP.头孢吡肟;CTX.头孢噻肟;CFX.头孢西丁;CAZ.头孢他啶;AZM.氨曲南;IMI.亚胺培南;MEM.美罗培南;GEN.庆大霉素;KAN.卡那霉素;AMI.阿米卡星;AZI.阿奇霉素;TET.四环素;MIN.米诺环素;DOX.多西环素;NAL.萘啶酸;CIP.环丙沙星;LEV.左氧氟沙星;GEM.吉米沙星;SXT.复方新诺明;CHL.氯霉素;S.敏感;I.中介;R.耐药

    Figure  1.  PFGE subtyping of 16 S. sonnei strains

    图  2  16株宋内志贺菌及23株参考菌株全基因组单核苷酸多态性聚类图

    注:红框内菌株为本次疫情分离菌株,其余为参考菌株

    Figure  2.  wgSNP subtyping of 16 S. sonnei and 23 reference strains

  • [1] 贾蕾, 吕冰, 田祎, 等. 北京市2008-2017年细菌性痢疾病原学监测分析[J]. 中华流行病学杂志,2019,40(2):165–169. DOI:10.3760/cma.j.issn.0254−6450.2019.02.008.

    Jia L, Lv B, Tian Y, et al. Pathogenic surveillance and related factors on bacillary dysentery in Beijing, 2008–2017[J]. Chin J Epidemiol, 2019, 40(2): 165–169. DOI: 10.3760/cma.j.issn.0254−6450.2019.02.008.
    [2] Qu M, Zhang X, Liu GR, et al. An eight-year study of Shigella species in Beijing, China: serodiversity, virulence genes, and antimicrobial resistance[J]. J Infect Dev Ctries, 2014, 8(7): 904–908. DOI:  10.3855/jidc.3692.
    [3] Bankevich A, Nurk S, Antipov D, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing[J]. J Comput Biol, 2012, 19(5): 455–477. DOI:  10.1089/cmb.2012.0021.
    [4] Aziz RK, Bartels D, Best AA, et al. The RAST server: rapid annotations using subsystems technology[J]. BMC Genomics, 2008, 9: 75. DOI: 10.1186/1471−2164−9−75.
    [5] Overbeek R, Olson R, Pusch GD, et al. The SEED and the rapid annotation of microbial genomes using subsystems technology (RAST)[J]. Nucleic Acids Res, 2014, 42(D1): D206–D214. DOI:  10.1093/nar/gkt1226.
    [6] Brettin T, Davis JJ, Disz T, et al. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes[J]. Sci Rep, 2015, 5(1): 8365. DOI:  10.1038/srep08365.
    [7] Price MN, Dehal PS, Arkin AP. FastTree: computing large minimum evolution trees with profiles instead of a distance matrix[J]. Mol Biol Evol, 2009, 26(7): 1641–1650. DOI:  10.1093/molbev/msp077.
    [8] Johansson MHK, Bortolaia V, Tansirichaiya S, et al. Detection of mobile genetic elements associated with antibiotic resistance in Salmonella enterica using a newly developed web tool: Mobile Element Finder[J]. J Antimicrob Chemother, 2021, 76(1): 101–109. DOI:  10.1093/jac/dkaa390.
    [9] Calvez S, Fournel C, Douet DG, et al. Pulsed-field gel electrophoresis and multi locus sequence typing for characterizing genotype variability of Yersinia ruckeri isolated from farmed fish in France[J]. Vet Res, 2015, 46(1): 73. DOI: 10.1186/s13567−015−0200−5.
    [10] Tenover FC, Arbeit RD, Goering RV, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing[J]. J Clin Microbiol, 1995, 33(9): 2233–2239. DOI: 10.1128/JCM.33.9.2233−2239.1995.
    [11] Holt KE, Baker S, Weill FX, et al. Shigella sonnei genome sequencing and phylogenetic analysis indicate recent global dissemination from Europe[J]. Nat Genet, 2012, 44(9): 1056–1059. DOI:  10.1038/ng.2369.
    [12] 李柏生, 陈柳军, 柯碧霞, 等. 广东省和广西壮族自治区部分地区2014-2016年宋内志贺菌病原学特征分析[J]. 中华流行病学杂志,2017,38(11):1541–1545. DOI:10.3760/cma.j.issn.0254−6450.2017.11.021.

    Li BS, Chen LJ, Ke BX, et al. Etiologic characteristics of Shigella sonnei strains isolated from some areas of Guangdong province and Guangxi Zhuang Autonomous Region of China, 2014–2016[J]. Chin J Epidemiol, 2017, 38(11): 1541–1545. DOI: 10.3760/cma.j.issn.0254−6450.2017.11.021.
    [13] 孟昭倩, 段然, 卜戈, 等. 一起由宋内志贺菌引起的寄宿学校细菌性痢疾暴发病原学和流行病学分析[J]. 中华流行病学杂志,2019,40(8):988–991. DOI:10.3760/cma.j.issn.0254−6450.2019.08.021.

    Meng ZQ, Duan R, Bu G, et al. Epidemiological and pathogenic features of a bacillary dysentery outbreak in a boarding school caused by Shigella sonneri[J]. Chin J Epidemiol, 2019, 40(8): 988–991. DOI: 10.3760/cma.j.issn.0254−6450.2019.08.021.
    [14] Shokoohizadeh L, Kaydani GA, Ekrami A. Molecular characterization of Shigella spp. isolates from a pediatric hospital in Southwestern Iran[J]. Gastroenterol Hepatol Bed Bench, 2017, 10(4): 319–322. DOI:  10.22037/ghfbb.v0i0.1201.
    [15] Seribelli AA, Frazão MR, Medeiros MIC, et al. Molecular typing and occurrence of beta-lactam resistance genes of Shigella sonnei strains isolated from 1983 to 2014 in the São Paulo state of Brazil[J]. Microbiol Immunol, 2017, 61(12): 547–553. DOI: 10.1111/1348−0421.12550.
    [16] Kozyreva VK, Jospin G, Greninger AL, et al. Recent outbreaks of shigellosis in California caused by two distinct populations of Shigella sonnei with either increased virulence or fluoroquinolone resistance[J]. mSphere, 2016, 1(6): e00344–16. DOI: 10.1128/mSphere.00344−16.
    [17] 侯凤琴, 沈宝铨, 孙新婷. 北京地区志贺痢疾杆菌耐药性研究[J]. 中国公共卫生,2000,16(2):157–158. DOI:10.3321/j.issn:1001−0580.2000.02.041.

    Hou FQ, Shen BQ, Sun XT. Research of drug resistance of Shigella spp. in Beijing[J]. China Public Health, 2000, 16(2): 157–158. DOI: 10.3321/j.issn:1001−0580.2000.02.041.
    [18] 鲍春梅, 陈素明, 郭桐生, 等. 对三代头孢菌素不敏感宋内志贺菌的流行趋势和克隆传播[J]. 中国抗生素杂志,2009,34(10):617–620, 636.

    Bao CM, Chen SM, Guo TS, et al. Epidemic tendency and clone transmission of insensitive Shigella sonnei against third generation cephalosporin[J]. Chin J Antibiot, 2009, 34(10): 617–620, 636.
    [19] 胡杰, 崔恩博, 鲍春梅, 等. 耐三代头孢菌素的肠道病原菌的增长趋势和耐药谱[J]. 实用检验医师杂志,2011,3(4):206–209. DOI:10.3969/j.issn.1674−7151.2011.04.004.

    Hu J, Cui EB, Bao CM, et al. Increasing tendency and resistant spectrum of enteric pathogens of resistant to third-generation cephalosporins[J]. Chin J Lab Pathol, 2011, 3(4): 206–209. DOI: 10.3969/j.issn.1674−7151.2011.04.004.
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  • 收稿日期:  2021-03-02

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