斯氏弓形杆菌基于基因组序列遗传特征分析

周贵兰; 王园园; 顾一心; 李颖; 鞠长燕; 王佳奇; 王海瑞; 陈晓丽; 段永翔; 张茂俊

doi:10.3784/jbjc.202010190355

斯氏弓形杆菌基于基因组序列遗传特征分析

Genetic characteristics of Arcobacter skirrowii based on whole genome sequence

摘要

摘要:
目的获得斯氏弓形杆菌的遗传特征，并对其耐药基因及毒力相关基因进行预测分析。
方法对本实验室分离培养的8株斯氏弓形杆菌进行基因组测序并从NCBI和PATRIC数据库中下载全部已完成基因组测序的15株斯氏弓形杆菌的全基因组序列，应用生物信息学软件对23株不同地域、不同宿主来源的斯氏弓形杆菌进行遗传特征分析。
结果 23株斯氏弓形杆菌中仅3株菌携带耐药基因，且均为β-内酰胺类抗生素耐药基因。所有菌株均含有与免疫逃避、侵袭及运动等相关的毒力基因。与其他菌种弓形杆菌常见的10种毒力基因比对分析发现，本研究中所有斯氏弓形杆菌菌株均含有ciaB、tlyA、cj1349、pldA和mviN基因，2株菌（CNAS12-1和F28）含有iroE基因，均不携带另外4种毒力基因。 21.7%（5/23）的菌株包含Ⅵ型分泌系统（T6SS）基因簇，且其与弯曲菌属内的T6SS存在基因组成及排列的差异。
结论斯氏弓形杆菌有较高的遗传多样性，部分菌株可能有潜在的耐药和致病特征。

Abstract:
Objective To understand the genetic characteristics of Arcobacter skirrowii from different sources and hosts and analyze their antibiotic resistance and virulence associated genes.
Methods Bioinformatics software was used for the comparative analysis on the whole genome sequences of 8 A. skirrowii strains isolated in our laboratory and the whole genome sequences of 15 A. skirrowii strains from NCBI and PATRIC database.
Results bla_OXA-464 was the only identified antibiotic resistance gene in 3 A. skirrowii strains. All the strains contained immune invasion, evasion and motility-related virulence genes. Compared with the 10 virulence genes identified in other Arcobacter, the virulence genes, ciaB, tlyA, cj1349, pldA and mviN were found in all of the tested strains. iroE was identified only in two strains (CNAS12-1 and F28), no other 4 virulence genes were found. Type Ⅵ secretion system (T6SS) gene cluster was found in 21.7% (5/23) of the tested strains, which had differed genetic constitution and arrangement compared with T6SS of other Arcobacter.
Conclusion A. skirrowii showed high genetic diversity, and some strains might have potential drug resistance and pathogenic characteristics.

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