我国西北地区蚊虫标本中广平病毒的检测

李樊 殷启凯 胡伟军 周莉薇 付士红 张少白 韩坤 聂凯 何英 许晓光 许松涛 梁国栋 王环宇

李樊, 殷启凯, 胡伟军, 周莉薇, 付士红, 张少白, 韩坤, 聂凯, 何英, 许晓光, 许松涛, 梁国栋, 王环宇. 我国西北地区蚊虫标本中广平病毒的检测[J]. 疾病监测.
引用本文: 李樊, 殷启凯, 胡伟军, 周莉薇, 付士红, 张少白, 韩坤, 聂凯, 何英, 许晓光, 许松涛, 梁国栋, 王环宇. 我国西北地区蚊虫标本中广平病毒的检测[J]. 疾病监测.
Li Fan, Yin Qikai, Hu Weijun, Zhou Liwei, Fu Shihong, Zhang Shaobai, Han Kun, Nie Kai, He Ying, Xu Xiaoguang, Xu Songtao, Liang Guodong, Wang Hunanyu. Detection of Quang Binh virus from mosquitoes in northwestern China[J]. Disease Surveillance.
Citation: Li Fan, Yin Qikai, Hu Weijun, Zhou Liwei, Fu Shihong, Zhang Shaobai, Han Kun, Nie Kai, He Ying, Xu Xiaoguang, Xu Songtao, Liang Guodong, Wang Hunanyu. Detection of Quang Binh virus from mosquitoes in northwestern China[J]. Disease Surveillance.

我国西北地区蚊虫标本中广平病毒的检测

基金项目: 国家自然科学基金(No. 31900156),宁夏回族自治区重点研发计划(No. 2019BCG01003), 中央引导地方项目(No. XZ202001YD0005C)
详细信息
    作者简介:

    李樊,女,吉林省白城市人,副研究员,主要从事虫媒病毒的分离鉴定和遗传变异研究,Email:lifan@ivdc.chinacdc.cn

    殷启凯,男,山东潍坊人,研究实习员,主要从事虫媒病毒病原学的相关研究,E-Mail:yinqk@ivdc.chinacdc.cn

    通讯作者:

    王环宇,Tel: 010-58900838,Email:wanghy@ivdc.chinacdc.cn

  • 中图分类号: R211;R373.3

Detection of Quang Binh virus from mosquitoes in northwestern China

Funds: This study were supported by the National Natural Science Foundation of China (No.31900156), and the fund for Key Research Project of Ningxia Science and Technology in 2019 (No.2019BCG01003), Local Science and Technology Project Guided by Central Government(XZ202001YD0005C)
More Information
  • 摘要:   目的   对我国西北地区蚊虫携带病毒开展调查,并快速发现重要的蚊媒病毒。  方法   通过形态学方法对采集蚊虫进行分类鉴定、深度测序和宏基因组分析方法分析蚊虫携带的病毒,结合Sanger测序法补全病毒基因组全序,应用系统进化分析方法分析病毒分子遗传特征。  结果   2019年7月采集自陕西省的中华按蚊标本和宁夏回族自治区的三带喙库蚊标本均检测到黄病毒属广平病毒(Quang Binh virus, QBV)。进化分析结果显示,这两株广平病毒序列与在中国辽宁省发现的广平病毒JM17156株具有较近亲缘关系,核酸序列同源性为99.8%。  结论  本研究首次在我国西北地区中华按蚊、三带喙库蚊标本中检测到广平病毒。
  • 图  1  基于SX1975和NX1976病毒株NS5区氨基酸序列的系统进化分析

    注:图中SX1975和NX1976株序列以实心圆表示。实分支节点上的校验值表示经1 000次重构后,该分支出现的概率;隐藏分支节点上小于70%的校验值

    Figure  1.  Phylogenetic tree based on NS5 region of amino acid sequences of SX1975 and NX1976 strains.

    表  1  宏基因组分析提取黄病毒相关序列

    Table  1.   Metagenomic analysis on extracted Flaviviridae related reads

    样本序号原始数据量(bp)读序数病毒相关读序病毒相关读序百分比(%)披膜病毒读序数布尼亚病毒读序数黄病毒读序数
    SX19752.8 G18.7 M51 36472.75155
    NX19763.1 G20.5 M25 1900.12652
    下载: 导出CSV

    表  2  广平病毒SX1975基因组核苷酸和氨基酸序列同源性分析

    Table  2.   Homology analysis of nucleotide and amino acid sequence between references and strain of SX1975

    序号病毒株1234567891011
    1SX19750.9730.9730.9980.9980.9430.8760.8750.6710.6710.610
    2QBV(NC_012671)0.8840.9950.9730.9740.9390.8790.8780.6790.6780.616
    3QBV(MH827523)0.8870.9720.9730.9740.9390.8800.8790.6800.6780.618
    4QBV(MH827524)0.9890.8830.8850.9980.9440.8760.8750.6720.6710.613
    5QBV(MG719525)0.9930.8840.8860.9860.9440.8770.8760.6720.6710.612
    6MFV(NC_021069)0.8120.8020.8050.8110.8130.8750.8740.6770.6760.614
    7MFV(AB981186)0.7300.7360.7350.7310.7330.7230.9940.6730.6720.622
    8MFV(LC513840)0.7310.7340.7340.7310.7330.7230.9850.6710.6700.623
    9CxFV(MH827522)0.5840.5780.5780.5840.5820.5870.5810.5830.9920.636
    10CxFV(MF582365)0.5830.5770.5770.5830.5820.5860.5800.5820.9850.638
    11CxFV(JQ518484)0.5530.5550.5530.5540.5520.5540.5520.5520.5610.562
      注: 核苷酸和氨基酸序列的同源性百分比分别显示在左下角和右上角。 QBV,Quang Binh virus. MFV,Mosquito Flavivirus. CxFV,Culex Flavivirus.
    下载: 导出CSV
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  • 收稿日期:  2021-06-23

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