Seroepidemiology of severe fever with thrombocytopenia syndrome bunyavirus in Jiangsu province
摘要: 目的 调查江苏省人与动物发热伴血小板减少综合征布尼亚病毒(SFTSV)血清流行病学情况,为控制疫情提供流行病学线索。 方法 2010年7-11月在江苏省南京江宁区、溧水县,常州溧阳市,无锡宜兴市,淮安盱眙县和连云港东海县共采集2853份血清,其中人血清1922份、动物(犬、羊、猪、牛、鹅、鼠和鸡)血清931份, 运用双抗原夹心ELISA法检测血清中SFTSV总抗体,并进行不同地区SFTSV总抗体阳性率比较。 结果 本次调查2853份血清样本中SFTSV总抗体阳性血清121份,阳性率为4.24%,提示江苏省存在SFTSV的流行。在7种被调查的动物样本中,5种动物(犬、羊、牛、猪、鸡)血清样本SFTSV总抗体阳性,阳性率分别为6.40%、57.14%、31.82%、5.33%和0.98%;人血清中SFTSV总抗体阳性率为0.94%。血清中SFTSV总抗体阳性率存在地区差异。人群与动物SFTSV血清总抗体阳性率存在正相关关系。 结论 江苏省是SFTSV的流行区域,需加强SFTSV的预防意识及诊断能力。Abstract: Objective To investigate the seroprevalence of severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) in human and animals in Jiangsu and provide scientific evidence for the development of prevention and control strategy. Methods A total of 2853 serum samples were collected in Lishui, Jiangning, Liyang, Yixing, Xuyi and Donghai in Jiangsu from July to November in 2010, including 1922 human serum samples and 931 animal serum samples (125 from dogs, 91 from goats, 225 from pigs, 88 from cattle, 50 from geese, 47 from mice, and 305 from chickens). A novel double antigen sandwich ELISA was conducted to detect the overall antibodies to SFTSV. The prevalence rates in different regions were compared with each other. Results SFTSV-specific antibodies were found in a total of 121 samples (4.24%), suggesting the wide circulation of SFTSV in the province. Of the 7 animal species tested, 5 species' sera, including dog, sheep, cattle, pig and chicken, were detected to be positive with the rates of 6.40%, 57.14%, 31.82%, 5.33% and 0.98% respectively. The prevalence rate in human was about 0.94%. The seroprevalence of SFTSV varied with regions and the positive correlation between human prevalence rate and animal prevalence rate was observed. Conclusion Jiangsu province is the endemic area of SFTSV infection, and it is necessary to strengthen the prevention and diagnosis of this emerging communicable disease.
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