Abstract: Objective To realize standardization of PFGE subtyping procedure for PulseNet China network laboratories. Methods The laboratory proficiency testing program was performed in 19 province/municipal centers for disease control and prevention which possessed standardized equipments. Results Up to 78.9% of the laboratories have passed the testing, and the data showed that the similarity of the patterns generated by the laboratories was 100%. Conclusion The laboratories passed the proficiency testing have sufficient capacity to perform the subtyping of pathogenic bacteria from their provinces and be included in PulseNet China.