Identification and tracing of norovirus GⅠ in epidemics of acute gastroenteritis in 6 schools in Xicheng district, Beijing, 2018−2019
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摘要:
目的 分析2018—2019年北京市西城区6所学校发生的急性胃肠炎疫情GⅠ诺如病毒的基因型别特征。 方法 收集2018—2019年西城区6所学校急性胃肠炎病例的粪便标本37份。 采用实时荧光定量反转录聚合酶链式反应(real-time RT-PCR)对提取的GⅠ和GⅡ诺如病毒核酸进行检测。 采用RT-PCR进行GⅠ诺如病毒聚合酶区和衣壳蛋白VP1区部分片段的扩增和测序。 用诺如病毒在线分型工具对测序成功的毒株进行初步鉴定和基因分型,用BioEdit 7.0.9.0软件进行序列比对,用MEGA 6.06 软件构建进化树。 结果 6所学校(18,S1~S4和19,S5~S6)发生的诺如病毒急性胃肠炎疫情均为聚集性疫情,GⅠ诺如病毒核酸阳性率为83.78%(31/37),GⅡ诺如病毒未检出;聚合酶区和衣壳蛋白VP1区进化分析分别显示,1起疫情的4株诺如病毒毒株分别与GⅠ.Pd参考株和GⅠ.3b参考株在同一分支,5起疫情的19株诺如病毒毒株与GⅠ.Pb参考株和GⅠ.6a参考株在同一分支;每所学校毒株相似性为100.0%。双区分型合并结果,1起疫情由GⅠ.Pd-GⅠ.3b引起,该重组株与GⅠ.3[P13]/Hu/MT008455/2018/Shanghai/CHI相似性高达99.6%;5起疫情由GⅠ.Pb-GⅠ.6a引起, 这重组株与GⅠ.6[P11]/Hu/MZ227264/2016/US相似性高达98.1%。 结论 2018—2019年北京市西城区6所学校诺如病毒急性胃肠炎聚集性疫情分别由GⅠ.Pd-GⅠ.3b和GⅠ.Pb-GⅠ.6a重组株引起。 应加强对诺如病毒重组株分子流行病学监测,为诺如病毒疫情提供预警预测的依据。 Abstract:Objective To analyze the genotype characteristics of norovirus GⅠ detected from the acute gastroenteritis epidemics in 6 schools in Xicheng district of Beijing from 2018 to 2019. Methods A total of 37 stool samples were collected from the acute gastroenteritis cases in the 6 schools during this period. Real-time fluorescent RT-PCR were used to detect the viral nucleic acids of norovirus GⅠ/GⅡ from the samples. The regions coding for RNA polymerase and capsid proteins VP1 of norovirus G I were amplified and sequenced in by RT-PCR. The successfully sequenced virus strains were preliminary identified and genotyped by using on-line Norovirus Typing Tool (Version 2.0). Software BioEdit7.0.9.0 was used for sequence alignment, and software MEGA 6.0 was used to construct phylogenetic tree. Results The acute gastroenteritis epidemics in 6 schools (18 S1−S4 and 19 S5−S6) were caused by norovirus. The positive rate of norovirus GⅠ nucleic acid was 83.78% (31/37), and no norovirus GⅡ was detected. The phylogenetic analysis on polymerase and capsid proteins VP1 region showed that 4 strains from 1 epidemic and GⅠ.Pd and GⅠ. 3b reference strains were in the same branch, and 19 strains from 5 epidemics and GⅠ.Pb and GⅠ.6a reference strains were in the same branch, the similarity of each school strain was 100.0%. The results of bipartition type showed that 1 epidemic was caused by the recombinant strain of GⅠ.Pd-GⅠ.3b, The similarity between GⅠ.Pd-GⅠ.3b and GⅠ. 3[P13]/Hu/MT008455/2018/Shanghai/chi was 99.6%, and 5 epidemics were caused by the recombinant strain of GⅠ.Pb-GⅠ.6a, the similarity between GⅠ.Pb-GⅠ.6a and GⅠ. 6[P11]/Hu/MZ227264/2016/US was 98.1%. Conclusion The epidemics of norovirus-induced acute gastroenteritis occurred in 6 schools in Xicheng district of Beijing from 2018 to 2019 were caused by GⅠ.Pd-GⅠ.3b and GⅠ.Pb-GⅠ.6a recombinant strain. It is necessary to strengthen the molecular epidemiological surveillance for norovirus recombinant strains to provide evidence for the early warning and prediction of norovirus infection epidemics. -
Key words:
- Norovirus /
- Acute gastroenteritis /
- Recombinant strains
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表 1 诺如病毒急性胃肠炎疫情检测结果概况
Table 1. Test results of epidemics of acute gastroenteritis caused by norovirus
学校(类型) 疫情发生时间
(年/月/天数)临床诊断
病例数罹患率
[%( n/N)]采集样本
件数实时荧光RT-PCR
阳性率
[%( n/N)]诺如病毒双区基因分型结果 在线分型 进化分型 毒株数 18 S1(小学) 2018/03/3 6 18.18(6/33) 4 100.00(4/4) GⅠ.Pd-GⅠ.3 GⅠ.Pd-GⅠ.3b 4 18 S2(小学) 2018/11/2 11 23.91(11/46) 7 71.43(5/7) GⅠ.Pb-GⅠ.6 GⅠ.Pb-GⅠ.6a 5 18 S3(中学) 2018/11/7 11 4.56(11/241) 6 66.67(4/6) GⅠ.Pb-GⅠ.6 GⅠ.Pb-GⅠ.6a 2 18 S4(小学) 2018/12/4 15 31.91(15/47) 5 100.00(5/5) GⅠ.Pb-GⅠ.6 GⅠ.Pb-GⅠ.6a 5 19 S5(小学) 2019/03/7 5 10.20(5/49) 4 75.00(3/4) GⅠ.Pb-GⅠ.6 GⅠ.Pb-GⅠ.6a 2 19 S6(小学) 2019/03/5 14 11.57(14/121) 11 90.91(10/11) GⅠ.Pb-GⅠ.6 GⅠ.Pb-GⅠ.6a 5 合计 62 11.55(62/537) 37 83.78(31/37) 2种重组株 23 注:毒株数为样本测序成功的毒株数量;RT-PCR. 反转录聚合酶链式反应 -
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