安徽省北方地区恙虫病血清学诊断和病原体基因序列分析

陈晴晴 张永根 刘一梅 史永林 龚磊 吴小珉 孙永

陈晴晴, 张永根, 刘一梅, 史永林, 龚磊, 吴小珉, 孙永. 安徽省北方地区恙虫病血清学诊断和病原体基因序列分析[J]. 疾病监测.
引用本文: 陈晴晴, 张永根, 刘一梅, 史永林, 龚磊, 吴小珉, 孙永. 安徽省北方地区恙虫病血清学诊断和病原体基因序列分析[J]. 疾病监测.
Chen Qingqing, Zhang Yonggen, Liu Yimei, Shi Yonglin, Gong Lei, Wu Xiaomin, Sun Yong. Serological diagnosis of tsutsugamushi disease and gene sequence analysis of Orientia tsutsugamushi in northern area of Anhui[J]. Disease Surveillance.
Citation: Chen Qingqing, Zhang Yonggen, Liu Yimei, Shi Yonglin, Gong Lei, Wu Xiaomin, Sun Yong. Serological diagnosis of tsutsugamushi disease and gene sequence analysis of Orientia tsutsugamushi in northern area of Anhui[J]. Disease Surveillance.

安徽省北方地区恙虫病血清学诊断和病原体基因序列分析

基金项目: 国家科技重大专项(No. 2018ZX10713003-002-005)
详细信息
    作者简介:

    陈晴晴,女,安徽省淮北市人,硕士,初级检验师,主要从事病原微生物检测工作,Email:cqq9966@163.com

    通讯作者:

    孙永,Tel:0551-64383112,Email:biosunyong@163.com

  • 中图分类号: R211;R376

Serological diagnosis of tsutsugamushi disease and gene sequence analysis of Orientia tsutsugamushi in northern area of Anhui

Funds: This study was supported by the National Science and Technology Major Project (No. 2018ZX10713003-002-005)
More Information
  • 摘要:   目的  对安徽省北方地区恙虫病临床诊断病例血清学检测分型和病原体基因序列分析,了解恙虫病东方体的基因型流行特征。  方法  收集2018年安徽省宿州市、蚌埠市、阜阳市临床诊断为恙虫病患者血液标本92份,采用间接免疫荧光和巢式聚合酶链反应分别检测病例血清中IgM抗体和血液中恙虫病东方体56 kDa型特异性抗原基因,并对扩增序列进行测序分析。  结果  92份临床诊断为恙虫病患者血液标本中IgM检测阳性61例,血清分型主要为Gilliam;核酸阳性48例,基因分型结果均为Kawasaki相关型。  结论  安徽省阜阳市、蚌埠市、宿州市恙虫病东方体基因型主要为Kawasaki相关型,存在暴发风险,需要加强对安徽各地区恙虫病东方体监测工作。
  • 图  1  恙虫病东方体tsa56基因系统发生树

    注:■代表本研究中PCR阳性序列;●安徽省已报导序列

    Figure  1.  Phylogenetic tree of Orientia tsutsugamushi constructed based on tsa56 gene

    表  1  恙虫病东方体tsa56基因扩增引物

    Table  1.   Primers for amplification of tsa56 gene of Orientia tsutsugamushi

    引物
    名称
    序列(5’-3’)聚合酶链
    反应反应
    参考
    文献
    P34TCAAGCTTATTGCTAGTGCAATGTCTGC第一轮8
    P55AGGGATCCCTGCTGCTGTGCTTGCTGCG
    P10GATCAAGCTTCCTCAGCCTACTATAATGCC第二轮
    P11CTAGGGATCCCGACAGATGCACTATTAGGC
    下载: 导出CSV
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  • 收稿日期:  2021-05-10

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