2011－2019年河北省柯萨奇病毒A组6型全基因组分析

刘莹莹; 谢赟; 于秋丽; 赵文娜; 苏通; 陆辉; 徐苗苗; 韩旭; 李琦

doi:10.3784/jbjc.202307130361

2011－2019年河北省柯萨奇病毒A组6型全基因组分析

Whole genome characteristics of coxsackievirus A6 in Hebei, 2011−2019

摘要

摘要:
目的分析河北省2011—2019年柯萨奇病毒A组6型（CVA6）肠道病毒的遗传进化及其非结构蛋白编码区（2A～2C，3A～3D）基因重组的情况。
方法收集河北省2011—2019年由CVA6所致手足口病的轻、重症病例样本进行病毒分离，采用肠道病毒全基因组通用扩增方法获得全基因序列。用MEGAX软件进行序列比对及系统进化分析，利用RDP4软件检测基因重组信号，利用SimPlot3.5.1验证基因重组事件。
结果通过进化分析可知，CVA6在2012年以前主要为D2亚型，2013—2019年主要为D3亚型。全基因组测序共得到13个分离株的完整序列，分别聚集于D3两个分支，同源性差异较大。重组分析显示，河北省CVA6 D3亚型的一个分支在2C区和P3区存在基因重组现象。
结论河北省CVA6肠道病毒存在基因重组现象，应完善CVA6肠道病毒全基因组序列数据库，对早期预警肠道病毒重组株引起本地区手足口病较大范围流行和暴发提供数据支持。

Abstract:
Objective To analyze the whole genome characteristics and evolutionary recombination events of coxsackievirus A6 (CVA6) in Hebei province during 2011−2019.
Methods The whole genome sequence of enterovirus was obtained by universal amplification of enterovirus genome. Enterovirus strains were isolated from mild and severe cases of hand, foot and mouth disease(HFMD) caused by CVA6. Sequence alignment and phylogenetic analysis were performed by using software MEGAX , gene recombination signals were detected by using software RDP4 , and gene recombination events were verified by using software package Sim Plots 3.5.1 .
Results The phylogenetic analysis indicated that the CVA6 isolates in Hebei mainly belonged to subtype D2 before 2012, and subtype D3 became predominant during 2013−2019. Whole genome sequencing obtained 13 complete sequences in two branches of 2C region and P3 region of the isolated subtype D3 strains.
Conclusion Gene recombination occurred in CVA6 isolated in Hebei . It is necessary to update the whole genome sequence database of CVA6 to provide data support for the early warning of possible large-scale epidemic of HFMD caused byrecombinant enterovirus strains in this area.

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