2013-2018年浙江省杭州市腹泻样本中O4血清群副溶血弧菌分子特征分析

俞骅 郑伟 陈琦 刘小东 郑之北 曹飞飞 汪皓秋

俞骅, 郑伟, 陈琦, 刘小东, 郑之北, 曹飞飞, 汪皓秋. 2013-2018年浙江省杭州市腹泻样本中O4血清群副溶血弧菌分子特征分析[J]. 疾病监测, 2022, 37(7): 931-934. doi: 10.3784/jbjc.202111260613
引用本文: 俞骅, 郑伟, 陈琦, 刘小东, 郑之北, 曹飞飞, 汪皓秋. 2013-2018年浙江省杭州市腹泻样本中O4血清群副溶血弧菌分子特征分析[J]. 疾病监测, 2022, 37(7): 931-934. doi: 10.3784/jbjc.202111260613
Yu Hua, Zheng Wei, Chen Qi, Liu Xiaodong, Zheng Zhibei, Cao Feifei, Wang Haoqiu. Molecular characteristics of clinical isolates of Vibrio parahaemolyticus serogroup O4 in Hangzhou, Zhejiang, 2013−2018[J]. Disease Surveillance, 2022, 37(7): 931-934. doi: 10.3784/jbjc.202111260613
Citation: Yu Hua, Zheng Wei, Chen Qi, Liu Xiaodong, Zheng Zhibei, Cao Feifei, Wang Haoqiu. Molecular characteristics of clinical isolates of Vibrio parahaemolyticus serogroup O4 in Hangzhou, Zhejiang, 2013−2018[J]. Disease Surveillance, 2022, 37(7): 931-934. doi: 10.3784/jbjc.202111260613

2013-2018年浙江省杭州市腹泻样本中O4血清群副溶血弧菌分子特征分析

doi: 10.3784/jbjc.202111260613
基金项目: 杭州市科技发展计划(No. 20191203B141);杭州市医学重点学科建设
详细信息
    作者简介:

    俞骅,男,浙江省宁波市人,副主任技师,主要从事食源性致病菌分子分型及基因组研究,Email:indexofire@gmail.com

    通讯作者:

    汪皓秋,Tel:0571–85176907,Email:wanghaoqiu@126.com

  • 中图分类号: R211; R378

Molecular characteristics of clinical isolates of Vibrio parahaemolyticus serogroup O4 in Hangzhou, Zhejiang, 2013−2018

Funds: This study was supported by the fund for Scientific and Technological Development Project in Hangzhou (No. 20191203B141) and Key Medical Discipline of Hangzhou
More Information
  • 摘要:   目的  分析浙江省杭州市腹泻样本中O4血清群副溶血弧菌分子特征。   方法  对2013—2018年杭州市腹泻样本中分离的1 061株副溶血弧菌进行血清凝集分析,采用qPCR方法检测其中147株O4血清群副溶血弧菌的耐热直接溶血素(tdh)、耐热相关溶血素(trh)、不耐热溶血素(tlh)和toxRS/new基因,并用脉冲场凝胶电泳(PFGE)方法进行分子分型。   结果  血清型为O4∶KUT(87.07%, 128/147)、O4∶K8(10.20%, 15/147)和O4∶K9(2.72%, 4/147)的菌株均为tdh+trh-tlh+toxRS/new-基因型。 根据PFGE图谱,菌株可以分成PA(以2016年后分离的菌株为主)和PB(2013—2018年菌株)2个簇,分别包含15和16个不同带型。 其中O4∶K8菌株均处于PA簇,O4∶K9菌株均处于PB簇,O4∶KUT菌株分布于PA和PB簇中。   结论  近年来杭州市分离自腹泻患者的O4∶KUT血清型菌株存在2个流行克隆群。 其中PB簇O4∶KUT菌株持续存在,PA簇O4∶KUT菌株在2016年出现并引起本地散在暴发,该型别菌株有成为地区流行克隆群的可能。
  • 图  1  147株副溶血弧菌的脉冲场凝胶电泳图谱聚类结果

    Figure  1.  Dendrogram of PFGE patterns of 147 V. parahaemolyticus isolates

    表  1  2013-2018年杭州地区腹泻患者来源副溶血弧菌血清型分布(株)

    Table  1.   Serotype distribution of V. parahaemolyticus isolated from diarrhea patients in Hangzhou, 2013−2018

    年份O3∶K6O4∶KUTO4∶K8O4∶K9其他总计
    20134319104985
    2014381710965
    2015736111495
    201618312618031358
    20171515212015230
    201814925151128228
    合计63724557161061 061
    下载: 导出CSV

    表  2  2013-2018年O3∶K6和O4∶KUT菌株来源患者的年龄和性别分布

    Table  2.   Sex and age distribution of patients infected with V. parahemolyticus O3∶K6 and O4∶KUT serotype, 2013−2018

    年龄组(岁)O3∶K6菌株来源病例数O4∶KUT菌株来源病例数
    男性女性总计男性女性总计
    <7134000
    7~169257310
    19~21621342910466170
    41~6078138212344
    >6014274551621
    合计307330637137108245
    下载: 导出CSV
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  • 收稿日期:  2021-11-26
  • 网络出版日期:  2022-04-13
  • 刊出日期:  2022-08-11

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