2018-2019年北京市呼吸道感染病例中肠道病毒D68型临床和流行特征研究

李爱华 龚成 李茂中 罗明 董梅 王怡婷 王雪 黄芳

李爱华, 龚成, 李茂中, 罗明, 董梅, 王怡婷, 王雪, 黄芳. 2018-2019年北京市呼吸道感染病例中肠道病毒D68型临床和流行特征研究[J]. 疾病监测, 2022, 37(6): 782-786. doi: 10.3784/jbjc.202109130497
引用本文: 李爱华, 龚成, 李茂中, 罗明, 董梅, 王怡婷, 王雪, 黄芳. 2018-2019年北京市呼吸道感染病例中肠道病毒D68型临床和流行特征研究[J]. 疾病监测, 2022, 37(6): 782-786. doi: 10.3784/jbjc.202109130497
Li Aihua, Gong Cheng, Li Maozhong, Luo Ming, Dong Mei, Wang Yiting, Wang Xue, Huang Fang. Clinical and epidemiological characteristics of enterovirus D68 in cases with respiratory infection in Beijing, 2018−2019[J]. Disease Surveillance, 2022, 37(6): 782-786. doi: 10.3784/jbjc.202109130497
Citation: Li Aihua, Gong Cheng, Li Maozhong, Luo Ming, Dong Mei, Wang Yiting, Wang Xue, Huang Fang. Clinical and epidemiological characteristics of enterovirus D68 in cases with respiratory infection in Beijing, 2018−2019[J]. Disease Surveillance, 2022, 37(6): 782-786. doi: 10.3784/jbjc.202109130497

2018-2019年北京市呼吸道感染病例中肠道病毒D68型临床和流行特征研究

doi: 10.3784/jbjc.202109130497
基金项目: 国家科技重大专项(No. 2017ZX10103004)
详细信息
    作者简介:

    李爱华,女,山东省阳信县人,硕士研究生,主管技师,主要从事传染病实验室检测和病原微生物研究工作,Email: liaihua1213@163.com

    通讯作者:

    黄芳,Tel:010–64407032,Email: hhffxdd@126.com

  • 中图分类号: R211; R373.2

Clinical and epidemiological characteristics of enterovirus D68 in cases with respiratory infection in Beijing, 2018−2019

Funds: This study was supported by the fund for National Science and Technology Major Project (No. 2017ZX10103004)
More Information
  • 摘要:   目的  分析北京市2018—2019年呼吸道感染病例中肠道病毒D68型(EV-D68)感染者的流行特征。  方法  利用北京市呼吸道病原体监测系统,收集2018—2019年全市35家哨点医院就诊的急性呼吸道感染患者的临床标本及流行病学资料,利用实时荧光PCR方法筛查肠道病毒和EV-D68,对EV-D68阳性标本进行VP1区序列的扩增、测序及进化分析。  结果  2018—2019年共收集急性呼吸道感染病例15 645例,检出肠道病毒阳性者467例(2.98%)。 22例(0.14%)EV-D68阳性病例中,<18岁组11例,18~60岁组7例,>60岁组4例;夏季(6—8月)检出7例,秋季(9—11月)11例,冬季(12月至次年2月)3例,春季(3—5月)1例;8例被诊断为上呼吸道感染,14例为肺炎(其中2例为重症肺炎);主要临床症状为发热(18/22,81.8%)、咳嗽(15/22,68.2%)。 14例EV-D68阳性经测序鉴定毒株属于亚型B3和新亚型D3,其中2018年有8例为B3亚型感染、1例为D3亚型感染,2019年5例均为D3亚型。  结论  EV-D68引起的呼吸道感染在北京市持续存在,主要在夏秋季流行,B3和新亚型D3为主要流行亚型。
  • 图  1  肠道病毒D68型VP1序列进化树

    注:▲. 本研究EV-D68毒株;●. 既往研究中2011-2017年北京地区EV-D68毒株;其他为不同国家不同年代各亚型代表株

    Figure  1.  Phylogenetic tree of EV-D68 strains based on VP1 gene region

    表  1  2018-2019年北京市急性呼吸道感染病例中肠道病毒及肠道病毒D68的检出情况

    Table  1.   Detections of enterovirus and EV-D68 in cases with acute respiratory tract infection in Beijing, 2018–2019

    年龄组(岁) 2018年 2019年
    病例数肠道病毒
    阳性数
    肠道病毒阳性
    率(%)
    EV-D68
    阳性数
    EV-D68阳性
    率(%)
    病例数肠道病毒
    阳性数
    肠道病毒阳性
    率(%)
    EV-D68
    阳性数
    EV-D68阳性
    率(%)
    0~2 3341154.93 80.34 2 503 923.6830.12
    18~2 772 702.53 40.142 553 843.2930.12
    >612 731 351.28 10.042 749 712.5830.11
    合计7 8372202.81130.17 7 8052473.1690.12
    下载: 导出CSV

    表  2  2018-2019年北京市急性呼吸道感染病例中肠道病毒D68阳性病例临床特征

    Table  2.   Clinical and epidemiological characteristics of EV-D68 positive cases with acute respiratory tract infection in Beijing, 2018−2019

    基本信息EV-D68阳性病例数构成比(%)
    年龄组(岁)
     0~1150.00
     18~731.82
     >60418.18
    性别
     男性1045.45
     女性1254.55
    诊断结果
     上呼吸道感染836.36
     肺炎(重症肺炎)1463.64
    临床症状
     发热1881.82
     咳嗽1568.18
     咳痰1045.45
     咽痛836.36
     呼吸困难313.64
     流涕627.27
     鼻塞29.09
     意识障碍418.18
     肌痛/关节痛418.18
     乏力418.18
     腹泻14.55
    影像学检查
     肺实变940.91
     胸腔积液29.09
    注:影像学检查结果百分比分别为22例阳性病例中出现肺实变和胸腔积液病例的比例
    下载: 导出CSV

    表  3  北京地区不同年份肠道病毒D68 VP1基因序列同源性分析

    Table  3.   Nucleotide and amino acid sequence homology of EV-D68 strains in Beijing

    亚型比较年份核苷酸序列
    同源性(%)
    氨基酸序列
    同源性(%)
    B32018和201495.80~97.6097.10~99.30
    2018和201697.10~98.7097.50~99.60
    201898.00~99.5098.90~100.00
    D32018—2019和201693.80~99.0098.20~99.60
    2018—201996.90~98.5098.50~100.00
    下载: 导出CSV
  • [1] Holm-Hansen CC, Midgley SE, Fischer TK. Global emergence of enterovirus D68: a systematic review[J]. Lancet Infect Dis, 2016, 16(5): e64–75. DOI: 10.1016/S1473−3099(15)00543−5.
    [2] Rahamat-Langendoen J, Riezebos-Brilman A, Borger R, et al. Upsurge of human enterovirus 68 infections in patients with severe respiratory tract infections[J]. J Clin Virol, 2011, 52(2): 103–106. DOI:  10.1016/j.jcv.2011.06.019.
    [3] Greninger AL, Naccache SN, Messacar K, et al. A novel outbreak enterovirus D68 strain associated with acute flaccid myelitis cases in the USA (2012-14): a retrospective cohort study[J]. Lancet Infect Dis, 2015, 15(6): 671–682. DOI: 10.1016/S1473−3099(15)70093−9.
    [4] Schieble JH, Fox VL, Lennette EH. A probable new human picornavirus associated with respiratory diseases[J]. Am J Epidemiol, 1967, 85(2): 297–310. DOI:  10.1093/oxfordjournals.aje.a120693.
    [5] Midgley CM, Jackson MA, Selvarangan R, et al. Severe respiratory illness associated with enterovirus D68 - Missouri and Illinois, 2014[J]. MMWR Morb Mortal Wkly Rep, 2014, 63(36): 798–799.
    [6] Meijer A, van der Sanden S, Snijders BEP, et al. Emergence and epidemic occurrence of enterovirus 68 respiratory infections in The Netherlands in 2010[J]. Virology, 2012, 423(1): 49–57. DOI:  10.1016/j.virol.2011.11.021.
    [7] Ikeda T, Mizuta K, Abiko C, et al. Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010[J]. Microbiol Immunol, 2012, 56(2): 139–143. DOI: 10.1111/j.1348−0421.2012.00411.x.
    [8] Zhang TG, Li AH, Chen M, et al. Respiratory infections associated with enterovirus D68 from 2011 to 2015 in Beijing, China[J]. J Med Virol, 2016, 88(9): 1529–1534. DOI:  10.1002/jmv.24505.
    [9] Wang GQ, Zhuge J, Huang WH, et al. Enterovirus D68 Subclade B3 strain circulating and causing an outbreak in the United States in 2016[J]. Sci Rep, 2017, 7(1): 1242. DOI: 10.1038/s41598−017−01349−4.
    [10] Dyrdak R, Grabbe M, Hammas B, et al. Outbreak of enterovirus D68 of the new B3 lineage in Stockholm, Sweden, August to September 2016[J]. Euro Surveill, 2016, 21(46): 30403. DOI: 10.2807/1560−7917.ES.2016.21.46.30403.
    [11] Knoester M, Schölvinck EH, Poelman R, et al. Upsurge of enterovirus D68, the Netherlands, 2016[J]. Emerg Infect Dis, 2017, 23(1): 140–143. DOI:  10.3201/eid2301.161313.
    [12] Tokarz R, Firth C, Madhi SA, et al. Worldwide emergence of multiple clades of enterovirus 68[J]. J Gen Virol, 2012, 93(9): 1952–1958. DOI: 10.1099/vir.0.043935−0.
    [13] McKay SL, Lee AD, Lopez AS, et al. Increase in acute flaccid myelitis - United States, 2018[J]. MMWR Morb Mortal Wkly Rep, 2018, 67(45): 1273–1275. DOI:  10.15585/mmwr.mm6745e1.
    [14] Xiang ZC, Gonzalez R, Wang Z, et al. Coxsackievirus A21, enterovirus 68, and acute respiratory tract infection, China[J]. Emerg Infect Dis, 2012, 18(5): 821–824. DOI:  10.3201/eid1805.111376.
    [15] Xiao QY, Ren L, Zheng SY, et al. Prevalence and molecular characterizations of enterovirus D68 among children with acute respiratory infection in China between 2012 and 2014[J]. Sci Rep, 2015, 5: 16639. DOI:  10.1038/srep16639.
    [16] Lu QB, Wo Y, Wang HY, et al. Detection of enterovirus 68 as one of the commonest types of enterovirus found in patients with acute respiratory tract infection in China[J]. J Med Microbiol, 2014, 63(3): 408–414. DOI: 10.1099/jmm.0.068247−0.
    [17] 张铁钢, 罗明, 李爱华, 等. 北京市一例重症肺炎儿童的呼吸道中检出肠道病毒D68[J]. 中华预防医学杂志,2015,49(3):282–283. DOI:10.3760/cma.j.issn.0253−9624.2015.03.019.

    Zhang TG, Luo M, Li AH, et al. The detection of enterovirus D68 from the specimen of a severe pneumonia case in Beijing[J]. Chin J Prev Med, 2015, 49(3): 282–283. DOI: 10.3760/cma.j.issn.0253−9624.2015.03.019.
    [18] Shen LY, Gong C, Xiang ZC, et al. Upsurge of enterovirus D68 and circulation of the new subclade D3 and subclade B3 in Beijing, China, 2016[J]. Sci Rep, 2019, 9(1): 6073. DOI: 10.1038/s41598−019−42651−7.
    [19] Huang WH, Wang GQ, Zhuge J, et al. Whole-genome sequence analysis reveals the enterovirus D68 isolates during the United States 2014 outbreak mainly belong to a novel clade[J]. Sci Rep, 2015, 5(1): 15223. DOI:  10.1038/srep15223.
    [20] 李飞, 沈军, 谭文杰. 肠道病毒D组68型流行情况研究进展[J]. 中国病毒病杂志,2015,10(6):471–475. DOI:10.16505/j.2095−0136.2020.0036.

    Li F, Shen J, Tan WJ. Recent progress in epidemiology of enterovirus D68[J]. Chin J Viral Dis, 2015, 10(6): 471–475. DOI: 10.16505/j.2095−0136.2020.0036.
    [21] Centers for Disease Control and Prevention. Clusters of acute respiratory illness associated with human enterovirus 68-Asia, Europe, and United States, 2008–2010[J]. MMWR Morb Mortal Wkly Rep, 2011, 60(38): 1301–1304.
    [22] Bal A, Sabatier M, Wirth T, et al. Emergence of enterovirus D68 clade D1, France, August to November 2018[J]. Euro Surveill, 2019, 24(3): 1800699. DOI: 10.2807/1560−7917.ES.2019.24.3.1800699.
    [23] Ikuse T, Aizawa Y, Yamanaka T, et al. Outbreak of enterovirus D68 among children in Japan-worldwide circulation of enterovirus D68 clade B3 in 2018[J]. Pediatr Infect Dis J, 2021, 40(1): 6–10. DOI:  10.1097/INF.0000000000002889.
    [24] Xiang ZC, Li LL, Ren LL, et al. Seroepidemiology of enterovirus D68 infection in China[J]. Emerg Microbes Infect, 2017, 6(5): e32.
    [25] Liu Y, Gong C, Luo M, et al. Seroepidemiology of enterovirus D68 in a healthy population in Beijing, China, between 2012 and 2017: A retrospective study[J]. J Med Virol, 2021, 93(6): 3524–3531. DOI:  10.1002/jmv.26132.
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  • 收稿日期:  2021-09-13
  • 网络出版日期:  2022-04-01
  • 刊出日期:  2022-07-14

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