张莉萍, 叶雪仪, 熊慧璋, 黄勇, 叶雪芬, 张巧利. 我国首例感染Y群脑膜炎奈瑟菌CC23病例的实验室分析[J]. 疾病监测, 2020, 35(7): 664-667. DOI: 10.3784/j.issn.1003-9961.2020.07.023
引用本文: 张莉萍, 叶雪仪, 熊慧璋, 黄勇, 叶雪芬, 张巧利. 我国首例感染Y群脑膜炎奈瑟菌CC23病例的实验室分析[J]. 疾病监测, 2020, 35(7): 664-667. DOI: 10.3784/j.issn.1003-9961.2020.07.023
Liping Zhang, Xueyi Ye, Huizhang Xiong, Yong Huang, Xuefen Ye, Qiaoli Zhang. Analysis on the first infection case of Neisseria meningitidis Y clonal complex 23 in China[J]. Disease Surveillance, 2020, 35(7): 664-667. DOI: 10.3784/j.issn.1003-9961.2020.07.023
Citation: Liping Zhang, Xueyi Ye, Huizhang Xiong, Yong Huang, Xuefen Ye, Qiaoli Zhang. Analysis on the first infection case of Neisseria meningitidis Y clonal complex 23 in China[J]. Disease Surveillance, 2020, 35(7): 664-667. DOI: 10.3784/j.issn.1003-9961.2020.07.023

我国首例感染Y群脑膜炎奈瑟菌CC23病例的实验室分析

Analysis on the first infection case of Neisseria meningitidis Y clonal complex 23 in China

  • 摘要:
    目的对广东省首例Y群流行性脑脊髓膜炎病例及其密切接触者进行病原学及分子分型分析。
    方法对分离自患者及171例密切接触者咽拭子的菌株,进行生化、血清鉴定、荧光聚合酶链式反应(PCR)及多位点序列分型(MLST)检测。
    结果生化、血清鉴定以及PCR检测结果均显示,该分离自患者的菌株为Y群脑膜炎奈瑟菌(Nm)菌株,MLST结果分析其属于ST-23克隆群(CC23),从171份密切接触者咽拭子中分离到1株Y群Nm,4株B群Nm。 Y群Nm属于CC23,4株B群Nm中1株为ST-11110型,无克隆群归类,2株可能属于ST-4821克隆群,1株为ST-5664,属于ST-4821克隆群。
    结论该病例为广东省首例Y群流脑病例,也是我国首例由Y群CC23引起的流脑病例,提示今后需进一步加强Y群流脑的病原学监测。

     

    Abstract:
    ObjectiveTo analyze the etiological and molecular characteristics of the first infection case of Neisseria meningitidis Y in Dongguan of Guangdong province.
    MethodsN. meningitidis strains were isolated from the patient’s cerebrospinal fluid and throat swabs of 171 close contacts by biochemistry test, serological grouping and fluorescence polymerase chain reaction (PCR). Genotypes of the isolates were analyzed using multi locus sequence typing (MLST).
    ResultsThe results of biochemistry test, serological test and real-time PCR showed that this strain from the patient was N. meningitidis Y. MLST results indicated that it belonged to ST-23 clonal complex (CC23). One strain of N. meningitidis Y and four strains of N. meningitidis B were isolated from 171 throat swabs of close contacts. The results of MLST indicated that the N. meningitidis Y belonged to CC23, one of the N. meningitidis B strain belonged to unassigned complex sequence types. Two N. meningitidis B strains might belong to ST-4821 clonal complex, while another one was identified as ST-5664 belonging to ST-4821 clonal complex.
    ConclusionThis was the first infection case of N. meningitidis Y in Guangdong as well as the first infection case of N. meningitidis Y CC23 in China, suggesting that the etiology surveillance for meningococcal meningitis caused by N. meningitidis Y should be further enhanced.

     

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