应用免疫捕获—逆转录—聚合酶链反应检测海域贝类中的甲肝病毒

俞晓进; 朱荣; 史智扬; 郭喜玲; 李显; 季国芳; 刘光中

doi:10.3784/j.issn.1003-9961.1998.6.225

应用免疫捕获—逆转录—聚合酶链反应检测海域贝类中的甲肝病毒

Study on Hepatitis A Virus in Shellfish Collected from the Jiangsu Coast by Immune Capture-Reverse Transcriptase-Polymerase Chain Reaction (IC-RT-PCR)

Yu Xiaojing et al,

摘要

摘要: 为了检测贝类标本中的甲型肝炎病毒，我们建立了敏感特异的免疫捕获—逆转录—聚合酶链反应技术（ＩＣ－ＲＴ－ＰＣＲ）。应用该技术检测不同地区采集的贝类标本中的ＨＡＶ－ＲＮＡ后发现：４６份贝类标本有２９份为阳性，检出率达６３.０４％（２９／４６），其中毛蚶为６３.１６％（１２／１９），文蛤为９０.００％（９／１０），蚶子为５４.５５％（６／１１），说明各种贝类有不同程度的污染；南部、中部地区贝类标本中的ＨＡＶ－ＲＮＡ检出率较高，北部地区１０份标本仅检出１份阳性，这与当地甲肝发病率较低一致。

Abstract: specimens of shellfish collected from 3 points of Jiangsu coast were detected for HAV RNA by using Immune Capture-Reverse Transcriptase-polymerase Chain Reaction (IC-RT-PCR)in 1993. 29 specimens of shellfish were positive for HAV RNA, the general positive rate was 63.04%(29/46). Of which, 63.16%(12/19) was found in Arca subcrenate, 90.0%(9/10)in Meretrix and 54.55%(6/11) in blood clam. So, it showed that many kinds of shellfish were contaminated by HAV in these areas of Jiangsu Province. The data also indicatedthat the positive rates of HAV RNA were 78.9% and 76.47% in both southern point A and middle point B, which were more higher than the north point C (only 10%). This pattern of contribution of HAV RNA was accorded with the contribution of HAV patients in Jiangsu Province.

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