Abstract: Objective As part of the UNICEF project on intensified routine immunization,the assessment was conducted to evaluate the knowledge,attitude and practice(KAP) on vaccination among the guardians of infants born in 2004 in four designated counties,providing evidence for the development of the promotion policy of the Expanded Programme on Immunization(EPI) in China.Methods In this assessment,the guardians of 852 infants born in 2004 received a house to house survey on vaccination.Results A total of 79.69% of the parents knew that infants should be vaccinated when born;69.48% of the guardians would have their children vaccinated positively;56.81% of them had taken their children to township hospitals for vaccination;47.89% of them knew that it was necessary to apply for an immunization certification after their children were vaccinated;and 49.30% of them also knew that the children's health status should be provided before vaccination.Conclusion The guardians' KAP on vaccination was not satisfying,for they had weak awareness of positive vaccination.It is suggested that specific EPI promotion policies and education for different groups of people should be developed and improved accordingly to have people take the initiative to participate in the immunization program.