Abstract: Objective　The present study was conducted to explore the relationship between leptin receptor (LEPR) gene Gln223Arg polymorphism and overweight-obesity, as well as multiple risk factors of overweight-obesity. Methods　A total of 177 normal weight subjects(control group) and 128 overweight-obese subjects were selected and arranged to take questionnaire, physical examination and blood biochemical tests. PCR-RFLP was applied to analyze the the polymorphism of LEPR gene Gln223Arg, and logistic regression was used for the analysis of the risk factors of overweight-obesity. Results　There was no significant difference of GG genotype and G allele of LEPR gene between the overweight-obesity group(72.7%, 84.0%) and the control group(66.7%, 80.2%). In each group, there was no significant difference for the level of age, systolic blood pressure(SBP), diastolic blood pressure (DBP), and the plasma level of total cholesterol(TC), triglyceride(TG), fasting blood glucose(FBG), high-density lipoprotein cholesterol(HDL-C), total protein(TP), albumin(ALB), uric acid(UA) and hemoglobin (Hb)between the two Gln223Arg genotypes, except waist circumference(WC) between the two Gln223Arg genotypes in the control group(P＜0.05). The logistic regression analysis showed that WC and low HDLC were the risk factors for overweight-obesity. Conclusion　The polymorphism of LEPR gene Gln223Arg may not be associated with overweight-obesity.