Abstract: ObjectiveTo investigate the HIV infection status and the behavior characteristics of the female sex workers in some places in Huaibei city of Anhui province. MethodsIn 2006, an anonymous questionnaire survey was conducted and blood samples were taken for HIV test among 405 female sex workers in sauna house, recreation club, barbershop, hotel and rented apartment. ResultsOne HIV-positive blood sample was found in 401 qualified samples (0.25%). The average age of female sex workers was 22.704.55 years old (ranged from 15-40 years old) and 42.72% of them were unmarried, 67.90% were graduated from junior middle school and 77.28% were local residents. The age of the first commercial sex was 19.182.60 years old (14-30 years old ). The average times of commercial sex in the latest week was13.766.06 ( 2-29 tomes). The condom use rate in the latest commercial sex was 87.65%. Rented apartments were with the highest exposure risk and the female sex workers changed their working places frequently. ConclusionThe rented apartments, the recreation club and the bathing spot were the places with high risk of AIDS transmission, it is necessary to conduct health education and behavior intervention in these places (especially in the rented apartments) in a deep going way to control the transmission of AIDS.