一种鉴别与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌的PCR方法

A PCR method of identification for Citrobacter freundii agglutinating with O157 serum

摘要: 目的利用基于O抗原翻转酶wzx基因的PCR方法，鉴别与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌和O157大肠埃希菌。方法用微生物鉴定仪对细菌进行生化鉴定，与O157血清凝集，同时扩增wzx基因。结果25株与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌及1株不与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌wzx基因扩增为阳性，8株与O157血清不凝集的弗氏枸橼酸杆菌wzx基因扩增为阴性，20株O157∶H7大肠埃希菌wzx基因扩增为阴性。结论对于可与O157血清发生强凝集的弗氏枸橼酸杆菌，基于O抗原翻转酶wzx基因的PCR方法是一种有效的快速鉴别方法。

Abstract: ObjectiveTo develop a PCR method to distinguish Citrobacter freundii which agglutinate with O157 serum from Escherichia coli O157. MethodsAll the strains isolated were subjects for biochemical identification, test for agglutination with O157 serum and test for wzx gene by PCR. ResultsThe wzx gene was detected in 25 strains of Citrobacter freundii which showed agglutination reaction with O157 serum，while no wzx gene was detected in 8 strains of Citrobacter freundii which showed no agglutination reaction with O157 serum. No wzxPCR product was detected in 20 strains of O157∶H7. ConclusionThe wzx-based PCR is a sensitive and rapid method to distinguish Citrobacter freundii strains which agglutinate with O157 serum from Escherichia coli O157 strains.