Abstract: Objective To understand the carriage and transmission status of the pathogen of hand foot and mouth disease (HFMD) among children in child care settings in Jian. Methods Epidemiological survey was conducted among the children in class with or without HFMD cases, and samples (including pharyngeal swabs and rectal swabs) were taken from them for enterovirus nucleic acid detection to identify virus type and calculate carriage rate. Result Five cases and 191 children in 3 child care settings were interviewed and their samples were detected, the overall virus carriage rate was 30.4%, which was 38.5% in classes with the cases, and 20.7% in classes without cases. Attributable risk (AR) was 46.2%, and communicable ratio was 3.9. The type of virus detected in the cases was same with the carriers in each child care setting. The positive rate of pharyngeal swabs was 9.3%, and the positive rate of rectal swabs was 26.7%. Conclusion The types of virus were different among 3 child care settings, Prompt interruption of virus transmission is the effective way to control HFND epidemic.