Abstract: Objective To understand the status of occupational HIV exposure and provide evidence to develop prevention measures in Guilin. Methods The information of occupational HIV exposure from 2004 to 2009 were collected to conduct statistical analysis on exposure cases occupation, exposure way and degree, exposure source degree, response method and outcome. Results Seventy four cases of occupational HIV exposure occurred during this period. Most cases were nurses (48.6%), polices (24.3%) and doctors (23.0%). The occupational HIV exposure cases increased by years, which was highest in 2009 and accounted for 32.4%. Most exposures were caused by needle puncture (51.4%), mucosa contamination (16.2%), scratch (14.8%) and wound contamination (10.8%). Most cases exposure degree and exposure source degree were grade 1 and mild, accounting 86.5% and 89.2% respectively. Of 74 cases,23 (31.1%) received correct local disinfection, the correct treatment rate among 14 severe exposure cases was 78.6%. Among the 14 cases for which preventive medicine should be taken,28.6% of them completed the full prevention course. The follow up for 74 cases indicated that no positive conversion of HIV antibody was found. Conclusion The medical workers and polices were the high risk groups of occupational HIV exposure. The rate of correct local disinfection was low among them. It is necessary to strengthen the education and training on prevention of occupational HIV exposure to reduce the exposure and increase the treatment level of the exposure.