喉癌患者穿通支原体检出及其肿瘤组织NF-κBp65表达
Detection of Mycoplasma penetrans and expression of NF-κBp65 in tumor tissue in laryngocarcinoma patients
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摘要: 目的 喉癌患者穿通支原体的检出,及其淋巴转移和肿瘤组织NF-κBp65表达分析,试图探讨穿通支原体感染与喉癌的发生、淋巴转移及其肿瘤组织NF-κBp65的表达之间相关性。 方法 采用分离培养和巢式聚合酶链反应检测93例喉癌和40例对照组中穿通支原体的存在,阳性标本进一步电镜确认,并对阳性和阴性患者进行淋巴转移及其肿瘤组织NF-κBp65表达分析。 结果 喉癌患者穿通支原体感染率明显高于非肿瘤组(PPP结论 穿通支原体感染与喉癌的发生、淋巴转移及其肿瘤组织NF-κBp65表达呈正相关。Abstract: Objective To detect Mycoplasma penetrans(Mpe) from laryngocarcinoma patients, understand the condition of lymphatic metastasis and the expression of NF-κBp65 in tumor tissue and explore the correlation between Mpe infection and the occurrence of laryngocarcinoma, between lymphatic metastasis and the expression of NF-κBp65 in tumor tissue. Methods Mpe detection was conducted for 93 laryngocarcinoma patients and 40 controls by isolation culture and nPCR, the Mpe-positive samples were confirmed by electron microscope. And the condition of lymphatic metastasis and the expression of NF-κBp65 in tumor tissue of Mpe-positive and Mpe-negative patients were analyzed. Results The Mpe infection rate of laryngocarcinoma patients was significantly higher than control group(PMpe-positive group was significantly higher than Mpe-negative group(PMpe-positive group was significantly higher than Mpe-negative group(PMpe-positive tumor tissue by electron microscope. Conclusion Positive correlation existed between Mpe infection and the occurrence of laryngocarcinoma and between lymphatic metastasis and the expression of NF-κBp65 in tumor tissue.
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