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张爽, 李颖, 张彦春, 马红梅, 王园园, 张赫. 2015-2017年北京市顺义区腹泻病例副溶血弧菌流行特征与分子分型特征分析[J]. 疾病监测, 2018, 33(5): 381-386. DOI: 10.3784/j.issn.1003-9961.2018.05.008
引用本文: 张爽, 李颖, 张彦春, 马红梅, 王园园, 张赫. 2015-2017年北京市顺义区腹泻病例副溶血弧菌流行特征与分子分型特征分析[J]. 疾病监测, 2018, 33(5): 381-386. DOI: 10.3784/j.issn.1003-9961.2018.05.008
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Zhang Shuang, Li Ying, Zhang Yanchun, Ma Hongmei, Wang Yuanyuan, Zhang He. Epidemiological and molecular characteristics of Vibrio Parahaemolyticus in Shunyi district of Beijing,2015-2017[J]. Disease Surveillance, 2018, 33(5): 381-386. DOI: 10.3784/j.issn.1003-9961.2018.05.008
Citation: Zhang Shuang, Li Ying, Zhang Yanchun, Ma Hongmei, Wang Yuanyuan, Zhang He. Epidemiological and molecular characteristics of Vibrio Parahaemolyticus in Shunyi district of Beijing,2015-2017[J]. Disease Surveillance, 2018, 33(5): 381-386. DOI: 10.3784/j.issn.1003-9961.2018.05.008
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2015-2017年北京市顺义区腹泻病例副溶血弧菌流行特征与分子分型特征分析

Epidemiological and molecular characteristics of Vibrio Parahaemolyticus in Shunyi district of Beijing,2015-2017

    摘要
  • 摘要: 目的 了解2015-2017年北京市顺义区副溶血弧菌（Vibrio parahaemolyticus）的流行特征和分子分型特征，为进一步防控工作提供依据。方法 分离2015-2017年顺义区腹泻病例标本中的副溶血弧菌，对分离株进行血清型和毒力基因检测，结合流行病学和临床资料进行分析。并将所得的O3：K6型副溶血弧菌菌株的脉冲场凝胶电泳（PFGE）图谱作聚类分析。结果 3年间，副溶血弧菌阳性检出率为8.68%（96/1 106），2份标本各检出2株副溶血弧菌，共获98株菌株；菌株分为9个血清型，O3：K6型最多（73.47%，72/98）；菌株均为tlh+trh-，仅1株tdh-，其余为tdh+；副溶血弧菌夏季检出率高，3年检出高峰均在8月；男女性副溶血弧菌阳性检出率差异无统计学意义（χ2=0.19，P=0.66）；副溶血弧菌阳性病例以16~45岁的青壮年居多，职业以其他职业、商业服务和工人居多；可疑感染食品多为水产类、蛋、肉及其制品；副溶血弧菌阳性病例症状以腹泻、脱水、腹痛为主，且多为水样便；大部分O3：K6型菌株的PFGE图谱聚集成簇，相似度较高，个别菌株相似度较低。结论 北京市顺义区副溶血弧菌感染引起的腹泻夏季高发，青壮年发病较多，水产及其制品为重要的可疑感染食品。O3：K6型为主要流行血清型，其PFGE图谱多聚集成簇。应加强夏季的防控工作。

     

    Abstract: Objective To understand the epidemiological and molecular characteristics of Vibrio parahaemolyticus in Shunyi district of Beijing from 2015 to 2017 and provide evidence for the prevention and control of related diseases. Methods V. parahaemolyticus was detected from the specimens collected from diarrhea patients in Shunyi during 2015-2017. Serotype and virulence gene detection of the isolates were carried,and epidemiological data and clinical data were analyzed. The pulsed field gel electrophoresis(PFGE) fingerprints of O3:K6 type V. parahaemolyticus strains were conduvted for cluster analysis. Results The detection rate of V. parahaemolyticus was 8.68%(96/1 106) during this period. Two strains of different serotypes were detected in each of 2 specimens respectively,thus 98 V. parahaemolyticus strains were detected. The detected strains belonged to 9 serotypes,most of them were O3:K6 type(73.47%,72/98). All strains were tlh positive,trh negative,only 1 straib was tdh negative,the remaining strains were tdh positive. The detection rate of V. parahaemolyticus was higher in summer than in other seasons. The annual detection peaks were all in August. The difference in detection rate had no significance between men and women(χ2=0.19,P=0.66). The majority of the infection cases were mainly young adults aged 16-45 years,and their occupations included commercial service,worker and others. The contaminated foods were mainly aquatic products,eggs, meat and their products. The symptoms of the infection cases were mainly diarrhea(watery stool),dehydration and abdominal pain. The PFGE fingerprints of most O3:K6 strains showed cluster and shared high similarity. Conclusion The incidence of diarrhea caused by V. parahaemolyticus was high in summer in Shunyi,and young adults were manly affected. Aquatic products were considered as the main infection sources. O3:K6 was the major circulating serotype,and most of their PFGE fingerprints showed cluster. It is necessary to strengthen the prevention and control of V. parahaemolyticus infection in summer.

     

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