CXCL1/CXCR2在羊瘙痒因子感染小鼠脑组织中的分布特征研究

周煜嘉; 陈嘉; 武月章; 石琦; 董小平; 陈操; 武瑞

doi:10.3784/jbjc.202210280469

CXCL1/CXCR2在羊瘙痒因子感染小鼠脑组织中的分布特征研究

Distribution characteristics of CXCL1/CXCR2 in brain tissue of scrapie infected mice

摘要

摘要:
目的探究朊病毒感染小鼠脑组织CXC趋化因子配体1 （CXCL1）与CXC趋化因子受体2 （CXCR2）的分布特征。
方法通过免疫组织化学、免疫组织荧光双染实验明确羊瘙痒因子139A及ME7感染终末期小鼠脑组织中CXCL1/CXCR2的分布特征，确定CXCL1/CXCR2的靶细胞及与羊瘙痒因子样朊蛋白（PrP^Sc）沉积的关系。
结果通过全脑区免疫组化染色发现，CXCL1/CXCR2在羊瘙痒因子139A及ME7感染终末期小鼠脑组织中的含量明显升高，主要分布在海马、皮层、丘脑、小脑及延髓5个脑区。 CXCL1与小胶质细胞和神经元细胞存在共定位，而CXCR2与神经元细胞存在共定位。在羊瘙痒因子139A及ME7感染终末期小鼠脑组织中CXCL1、CXCR2和PrP^Sc三者存在明显共定位。
结论 CXCL1/CXCR2分布于朊病毒感染小鼠脑组织中朊病毒病理特征集中的脑区。

Abstract:
Objective To explore the distribution characteristics of C-X-C motif chemokine ligand 1 (CXCL1) and CXC chemokine receptor 2 (CXCR2) in brains of prion infected mice.
Methods The distribution characteristics of CXCL1/CXCR2 in the brain tissues of scrapie agents 139A and ME7 infected mice at end stage were determined by immunohistochemistry and immunofluorescence double staining, and the target cells of CXCL1/CXCR2 and its correlation with PrP^Sc deposition were determined.
Results The results of immunohistochemical staining of the whole brain indicated that CXCL1/CXCR2 significantly increased in the brain tissues of mice infected with scrapie agents 139A and ME7, mainly distributed in the hippocampus, cortex, thalamus, cerebellum and medulla oblongata. CXCL1 co-localized with microglia and neuronal cells, while CXCR2 co-localized with neuronal cells. CXCL1, CXCR2 and PrP^Sc showed obvious co-localization in the brain tissues of mice infected with scrapie agents 139A and ME7.
Conclusion CXCL1/CXCR2 is distributed in brain regions with prion pathological features in prion infected mice.

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