Abstract: The diseases caused by Mycobacteria include tuberculosis (TB), non-tuberculous mycobacterial diseases and leprosy, in which TB is the most serious and infectious one. TB is one of major global infectious diseases that seriously endanger human health and life and one of the major diseases in disease prevention and control in China. Through coughing, expectoration, sneezing, etc., the “droplet nucleus” carrying Mycobacterium tuberculosis is spread from infectious TB patients, causing infection in people nearby. Therefore, the prevention of airborne transmission of TB in health care institutions, public places, and even in families is one of the key measures in TB prevention and control. Active mist ions are charged groups with strong oxidation, including hydroxyl radicals, superoxide radicals, superoxide hydrogen radicals, etc. Under the package and protection of water molecules, they have better stability in air. Disinfection evaluation experiments have showed that active mist ion has good disinfection and sterilization effects on various bacteria and viruses, including Mycobacteria. The experts invited by Chinese Anti-tuberculosis Association, Zoonotic Tuberculosis Sub-association of Chinese Anti-tuberculosis Association and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention have reached an consensus on the technical principle of active mist ion in air disinfection , composition of disinfection factors, disinfection effect and application in Mycobacterium disinfection through data inquiry, discussion and experimental research to provide technical guidance for the standardized application of active mist ion in air disinfection.