4种弯曲菌国家标准株实验室评价及应用分析

王海瑞; 顾一心; 陈晓丽; 周贵兰; 张鑫; 张茂俊

doi:10.3784/jbjc.202307100329

4种弯曲菌国家标准株实验室评价及应用分析

Laboratory evaluation and application analysis for four national standard Campylobacter strains

摘要

摘要:
目的依据《病原微生物菌（毒）种国家标准株评价技术标准》（WS/T 812—2022），对空肠弯曲菌、结肠弯曲菌、乌普萨拉弯曲菌和红嘴鸭（海鸥）弯曲菌4个菌种标准菌株病原特征进行实验室验证，评价应用标准菌株作为参比菌株进行弯曲菌检验标准实验室检测的可行性。
方法通过形态观察、生化试验及细菌动力学试验等方法获得标准菌株的菌落特点、形态特征、重要生化表型特征及运动特点；通过基因组测序获得菌株基因组水平遗传特征；通过琼脂扩散法和E-test法检测标准菌株的抗生素敏感性特征；通过空肠弯曲菌和结肠弯曲菌检验标准进行标准菌株作为参比菌种的应用评价。
结果标准菌株空肠弯曲菌NPRC（S） 01.13897、结肠弯曲菌NPRC（S） 01.13898、乌普萨拉弯曲菌NPRC（S） 01.13899和红嘴鸭弯曲菌NPRC（S） 01.13900均符合弯曲菌对应菌种的病原特征，可以作为弯曲菌检验的参比菌株。
结论本研究中的标准菌株具有相应菌种的病原特征，可作为弯曲菌检测及菌种鉴定的参比菌株。

Abstract:
Objective To obtain the pathogenic characteristics of four standard strains of Campylobacter jejuni, Campylobacter coli, Campylobacter upsaliensis and Campylobacter lari, and evaluate the results of isolation and identification for Campylobacter detection with the standard methods using these strains as references according to the Technical Requirements for Evaluation of National Standard Strains of Pathogenic Microorganisms(WS/T 812—2022).
Methods The colony on the medium, morphological, biochemical, and motility characteristics were investigated by the standard and universally accepted methods. The genomic profiles of these strains were obtained by genome sequencing and bioinformatic analysis. The antibiotic susceptibility of these strains was tested by agar diffusion and E-test methods. The application of these four strains as reference was evaluated by the detection standards of C. jejuni and C. coli.
Results The characteristics of C. jejuni NPRC(S) 01.13897, C. coli NPRC(S) 01.13898, C. upsaliensis NPRC(S) 01.13899, and C. lari NPRC(S) 01.13900 were well consistent with the related species of Campylobacter. Higher specificity and sensitivity were obtained as references for Campylobacter detection.
Conclusion These four standard strains hold the characteristics of corresponding species and could be used as reference strains for Campylobacter isolation and identification.

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