Abstract: In November 2022, the National Health Commission issued the Technical Requirements for Evaluation of National Standard Strains of Pathogenic Microorganisms (WS/T 812—2022), which clarified the definition of national standard strains of pathogenic microorganisms, and made unified technical requirements for the evaluation indexes of national standard strains of pathogenic microorganisms. The basic framework of the evaluation procedure for national standard strains of pathogenic microorganisms was established. To carry out the evaluation of national standard strains of pathogenic microorganisms and promote them to support actual work needs, it is necessary to develop technical procedures such as work instructions to elaborate the operation process of the evaluation on the strains, which enables people to understand the requirements for the strains certification including bacterial (virus) strains review and identification, evaluation team formation, data file review, meeting evaluation, and evaluation report. The evaluation technical procedure will become an important supplement and expansion of our national standard system of pathogenic microorganism resource preservation, which will promote the application of national standard strains and improve the overall quality of our pathogenic microorganism resource.