一步法快诊霍乱技术现场应用的效果观察

An observation on rapid detection of vibrio cholerae in field trials

摘要: 本文报告“霍乱一步法快诊试剂”在国内部分地区现场应用及与常规分离鉴定法的比较结果。共观察了 79份粪便标本直接使用“一步法快诊”的检测结果及 47份标本增菌 6 - 8小时后同样用该法的检测结果。现场考查表明 ,一步快诊法适用于检查急性期病人 ,从粪便标本中不仅能查出活菌 ,还能检查就诊前服用过抗菌药物的患者 ,其检出率高于常规培养法 (χ2 =17.2 5P<0.005 ) ;而增菌后标本的一步快诊法能得到和常规法完全相同的特异性与敏感性 ,可用于检定轻型病人和健康带菌者 ,但比常规法简便、快速。

Abstract: stool specimens of diarrheal cases using direct rapid test for detection of vibrio cholerae were reported.The results showed that the positive rate of direct rapid test in the acute period cases was higher than routine method(χ2=17.25 P<0.005).Although the specificity of this method was same as the routine,that the sensitivity was higher than the routine one and it has an advantage of simiplicity.