Abstract: Objective To understand the immunity level of hepatitis B in healthy population in Taiyuan and provide evidence for the reduction of infection rate and development of preventive and control measures. Methods According to national serological survey protocol for hepatitis B and other diseases, healthy people aged 3-59 years were selected in Taiyuam by probability proportion to size (PPS) sampling. Enzyme linked immunosorbent assay (ELISA) was conducted to detect HBsAg and HBsAb. Results The positive rate of HBsAg was 1.5%, and the positive rate of HBsAb was 44.3% in healthy population, which varied by areas. The differences on HBsAg and HBsAb had statistical significance in different age groups, but had no statistical significance between males and females. There were significant differences on immunity level in people with different occupation and different immunization history. Conclusion hepatitis B vaccination is the most effective measure to prevent hepatitis B. It is suggested to strengthen the management of vaccination and take targeted measures to increase the positive conversion rate of hepatitis B antibody.