Detection of norovirus genotype I and Ⅱ in diarrhea case samples by real-time  RT-PCR assay

ObjectiveTo set up a real-time-PCR assay for the detection of norovirus RNA in stool samples from diarrhea cases in suspected norovirus infection epidemic, provide reliable and accurate technical support for the epidemic control. MethodsThe RNA extracted from 18 stool samples were detected for the norovirus nucleic acid by real-time-PCR. ResultsNorovirus nucleic acid was detected in 13 of 18 stool samples with the positive rate of 72.2%. Of 13 positive samples, 6 were norovirus genotype I positive, 6 were norovirus genotype Ⅱ positive, 1 was both genotype I and genotype Ⅱ positive. ConclusionThe real-time-PCR could be used to detect norovirus RNA and provide accurate and reliable results.