Prepartion of the Medium for Isolation of Simulated Samples of V．Cholear in Winter Condition

In order to study the mechanigrn of over witer of V．Cholera．the preparation of the mediam for isolating the simulated samples of V．Cholera in witer condition was carried out V．eholra was difficult to isolate in winter season because of the bacterium might be changed in bi-ological characteristics due to the cold stimulation．Some substances related to the activities of enyyme such as coeyymes，prosthetic groups and growth stimulating substances were supplement-ed into the medium and maintained high osmotic pressure condition as well．The isolating rate of this so called Hypertonic Camosine-Tween Medium(HCTM)was 53～73％。while the control～gentamicin medium was only 13％．