Early Diagnosis of Leptospirosis by Using Immuno Gold Silver Staining and Reflection Contrast Microscopy

73 blood samples collected from patients were detected by immuno Gold Silver Staining and Reflection Contrast Microscopy (IGSS/RCM).9 of these samples were positive by IGSS/RCM.Positive rate was 12.3%.Of 45 amples from those patients ,their onset dates were within 3 days,8 samples were positive and positive rate was 17.8% by IGSS/RCM.There was no difference between the rates by using this mothod and blood culture method,but was higher than Microscopy Agglutination Test(MAT).The authors suggested that IGSS/RCM was an ideal method for early diagnosis of Leptospirosis.In contrast with culture and MAT,it was simple,rapid and sensitive.