Study on 16S rRNA Gene Typing of Vibrio Cholerae Isolated From Zhejiang Province

Using the probe of 16S Rrna gene, we investigated the 16S Rrna gene restriction patterns (16S Rran GRP) of 88 strains of Vibrio cholerae isolated from Zhejiang province after their chromosomes were digested with BglⅠ. Results showed that the fragments containing 16S Rrna were sized 2-21Kb in 16S Rrna GRP of BglⅠ. 88 strains could be divided into 9 ribotypes (RT), 6 ribotypes were in EVC and 3 ribotypes in VC O139. It was found that the variance of ribotype was probably the cause of new epidemic. Combining with ct gene detection,Vc 0139 was similar to EVC in hereditary characteristies,but there was also some differece between them.