The Standardization of MAT for the Detection of Leptospirosis Antibody and International Testing of theVeracity for MAT Results

Objective The present study was designed to standardize the traditional MAT methods used for the detection of leptospirosis antibody for the improvement of the reliability of test results，allowing the comparision and analysis of the MAT results obtained in different laboratories．Methods On the base of the methods newly recommended by WHO/ILS，The procedures involved in MAT currently implemented in China，including the preparation of culture medium、leptospira concentration、antigen preparation、serum inactivating，the time and temperature for antigen-antibody reaction and the criterions for the evaluation of result etc．Were standardized．Results Each of the blind samples (encoded as A,B,C,D and E) provided by WHO/ILS was repeatedly detected for three times and the results were the same．And the results were reported to WHO/ILS for confirmation and it was reported that the detected serogroup results of the five samples were accurate，the antibody titres consistent with the standards．Conclusions The standardized procedure of MAT for the detection of leptospirosis allows for more steady and reliable results，testing the exact serogroup and titre of serum leptospirosis antibody perfectly in the international MAT scheme．Standardized MAT contributes the improvement of the serological surveillance of leptospirosis in our country and is worthy of application．