Etiological survey on a diarrhea outbreak epidemic caused by water source contaminated by Salmonella paratyphi A

Objective An etiological survey was conducted on a diarrhea outbreak epidemic to make clear of the cause and implement effective prevention and control．Methods Fluorescence PCR rapid detection，normal etiological separation and cultivation，phagelysis expedments，ATB automated identification system were used to make etiological detection and identification on prob-able and suspected cases，environmental water source and drinking water source samples．Results Through fluorescence PCR detection and etiological separation，cultivation and identification，in suspected samples from patient feces，blood，water source and drinking water，10 samples were Salmonella paratyphi A positive with 83.33％ detection rate，in which 4 samples from feces，4 samples from blood．One sample from water source and one sample from drinking water．The ten isolates from different sources showed the same serological subtype，biochemical reaction，drug sensitivity and phage lysis experiments of Salmonella paratyphi．Conclusion This was a diarrhea outbreak epidemic caused by water source contaminated by Salmonella paratyphi A．The detection of this bacterium added a new type of etiological bacteria in diarrhea etiological mapping in this district