Genotyping of 99 clinical isolates of Mycobacterium tuberculosis using spoligotyping in Hangzhou

Objective　The present study was conducted to identify the genotypes and species of Mycobacterium tuberculosis(M.Tuberculosis) strains isolated in Hangzhou, the spacer oligonucleotide typing (Spoligotyping) was employed in the present study, providing evidence for the prevention and management of tuberculosis (TB). Methods　M. tuberculosis clinical strains isolated from patients with tuberculosis (TB) in Hangzhou were collected and genotyped with spoligotyping. Gene cluster analysis was performed using BioNumerics (Version 5.0) software. Results　A total of 99 clinical isolates of M. tuberculosis were collected in Hangzhou, which were divided into the Beijing family and the non-Beijing family by means of Spoligotyping, accounting for 64.65%(64/99) and 35.35%(35/99), respectively. A total of 20 genotypes were found, including 9 unique ones and11 clusters that contained the other 90 strains. As to the Beijing family, 89.06%(57/64) of the strains belonged to the typical Beijing family; the 35 strains from the non-Beijing family could be subdivided into 16 genotypes with 10 unique ones. Conclusion　Strong allelic diversity of M. tuberculosis in Hangzhou was found. The Beijing family predominated, though polymorphism in the non-Beijing family was more remarkable.